Pronounced somatic bottleneck in mitochondrial DNA of human hair

Alison Barrett, Barbara Arbeithuber, Arslan Zaidi, Peter Wilton, Ian M. Paul, Rasmus Nielsen, Kateryna D. Makova

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Heteroplasmy is the presence of variable mitochondrial DNA (mtDNA) within the same individual. The dynamics of heteroplasmy allele frequency among tissues of the human body is not well understood. Here, we measured allele frequency at heteroplasmic sites in two to eight hairs from each of 11 humans using next-generation sequencing. We observed a high variance in heteroplasmic allele frequency among separate hairs from the same individual-much higher than that for blood and cheek tissues. Our population genetic modelling estimated the somatic bottleneck during embryonic follicle development of separate hairs to be only 11.06 (95% confidence interval 0.6-34.0) mtDNA segregating units. This bottleneck is much more drastic than somatic bottlenecks for blood and cheek tissues (136 and 458 units, respectively), as well as more drastic than, or comparable to, the germline bottleneck (equal to 25-32 or 7-10 units, depending on the study). We demonstrated that hair undergoes additional genetic drift before and after the divergence of mtDNA lineages of individual hair follicles. Additionally, we showed a positive correlation between donor's age and variance in heteroplasmy allele frequency in hair. These findings have important implications for forensics and for our understanding of mtDNA dynamics in the human body.

Original languageEnglish (US)
Article number20190175
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume375
Issue number1790
DOIs
StatePublished - Jan 1 2020

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Mitochondrial DNA
Hair
hairs
mitochondrial DNA
Gene Frequency
gene frequency
Tissue
Cheek
Blood
Human Body
Mitochondrial Dynamics
Genetic Drift
hair follicles
Hair Follicle
blood
Population Genetics
genetic drift
Embryonic Development
confidence interval
population genetics

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Barrett, Alison ; Arbeithuber, Barbara ; Zaidi, Arslan ; Wilton, Peter ; Paul, Ian M. ; Nielsen, Rasmus ; Makova, Kateryna D. / Pronounced somatic bottleneck in mitochondrial DNA of human hair. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 2020 ; Vol. 375, No. 1790.
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Pronounced somatic bottleneck in mitochondrial DNA of human hair. / Barrett, Alison; Arbeithuber, Barbara; Zaidi, Arslan; Wilton, Peter; Paul, Ian M.; Nielsen, Rasmus; Makova, Kateryna D.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 375, No. 1790, 20190175, 01.01.2020.

Research output: Contribution to journalArticle

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