Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors

Raymond Judware, Thomas S. McCormick, Susanne Mohr, Jong Yun, Eduardo G. Lapetina

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ligation of integrins to an extracellular matrix activates signal transduction systems which produce multiple responses in different cell types. Adhesion often provides a survival signal to cells; disruption of adhesion frequently results in apoptosis. Our laboratory has utilized apoptosis-sensitive and -resistant cell lines to investigate the role of integrin expression and function in regulation of apoptosis in macrophages. Chronic exposure of murine macrophage-like RAW264.7 cells to apoptosis inducing agents (bacterial lipopolysaccharide and interferon-γ) resulted in the generation of a derivative cell line (RES) resistant to apoptosis. Observation of RAW and RES cultures indicated a difference in adhesion between the two cell types. The two cell lines also exhibit significant differences in expression of integrins previously characterized to be important in apoptosis.

Original languageEnglish (US)
Pages (from-to)507-512
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number2
DOIs
StatePublished - May 19 1998

Fingerprint

Macrophages
Integrins
Apoptosis
Adhesion
Cells
Cell Line
Signal transduction
Interferons
Extracellular Matrix
Ligation
Lipopolysaccharides
extracellular matrix receptor
Signal Transduction
Observation
Derivatives

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{86818e69810d4061a5866e62b969030d,
title = "Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors",
abstract = "Ligation of integrins to an extracellular matrix activates signal transduction systems which produce multiple responses in different cell types. Adhesion often provides a survival signal to cells; disruption of adhesion frequently results in apoptosis. Our laboratory has utilized apoptosis-sensitive and -resistant cell lines to investigate the role of integrin expression and function in regulation of apoptosis in macrophages. Chronic exposure of murine macrophage-like RAW264.7 cells to apoptosis inducing agents (bacterial lipopolysaccharide and interferon-γ) resulted in the generation of a derivative cell line (RES) resistant to apoptosis. Observation of RAW and RES cultures indicated a difference in adhesion between the two cell types. The two cell lines also exhibit significant differences in expression of integrins previously characterized to be important in apoptosis.",
author = "Raymond Judware and McCormick, {Thomas S.} and Susanne Mohr and Jong Yun and Lapetina, {Eduardo G.}",
year = "1998",
month = "5",
day = "19",
doi = "10.1006/bbrc.1998.8650",
language = "English (US)",
volume = "246",
pages = "507--512",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors. / Judware, Raymond; McCormick, Thomas S.; Mohr, Susanne; Yun, Jong; Lapetina, Eduardo G.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 2, 19.05.1998, p. 507-512.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Propensity for macrophage apoptosis is related to the pattern of expression and function of integrin extracellular matrix receptors

AU - Judware, Raymond

AU - McCormick, Thomas S.

AU - Mohr, Susanne

AU - Yun, Jong

AU - Lapetina, Eduardo G.

PY - 1998/5/19

Y1 - 1998/5/19

N2 - Ligation of integrins to an extracellular matrix activates signal transduction systems which produce multiple responses in different cell types. Adhesion often provides a survival signal to cells; disruption of adhesion frequently results in apoptosis. Our laboratory has utilized apoptosis-sensitive and -resistant cell lines to investigate the role of integrin expression and function in regulation of apoptosis in macrophages. Chronic exposure of murine macrophage-like RAW264.7 cells to apoptosis inducing agents (bacterial lipopolysaccharide and interferon-γ) resulted in the generation of a derivative cell line (RES) resistant to apoptosis. Observation of RAW and RES cultures indicated a difference in adhesion between the two cell types. The two cell lines also exhibit significant differences in expression of integrins previously characterized to be important in apoptosis.

AB - Ligation of integrins to an extracellular matrix activates signal transduction systems which produce multiple responses in different cell types. Adhesion often provides a survival signal to cells; disruption of adhesion frequently results in apoptosis. Our laboratory has utilized apoptosis-sensitive and -resistant cell lines to investigate the role of integrin expression and function in regulation of apoptosis in macrophages. Chronic exposure of murine macrophage-like RAW264.7 cells to apoptosis inducing agents (bacterial lipopolysaccharide and interferon-γ) resulted in the generation of a derivative cell line (RES) resistant to apoptosis. Observation of RAW and RES cultures indicated a difference in adhesion between the two cell types. The two cell lines also exhibit significant differences in expression of integrins previously characterized to be important in apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=0032546469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032546469&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.8650

DO - 10.1006/bbrc.1998.8650

M3 - Article

C2 - 9610392

AN - SCOPUS:0032546469

VL - 246

SP - 507

EP - 512

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -