Properties of human epithelioid cells established in vitro by a herpesvirus [ibrv(Hmc)] isolated from cytomegalovirus-transformed human cells

Laszlo Geder, Roger Ladda, John W. Kreider, Edgar J. Sanford, F. Rapp

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Infectious bovine rhlnotracheitis virus [IBRV- (HMC)], a double-enveloped herpesvirus, was Isolated from human embryo lung fibroblasts transformed by cytomegalovirus (CMV). This agent was identified as an IBRV strain that was antigenically related to human CMV. Inoculation of a primary human kidney cancer cell culture with IBRV(HMC) resulted In persistent Infection and subsequent establishment of a cell line [IBRV(HMC)HKC-1]. Virus-related nuclear, cytoplasmic, and cell membrane antigens were detected in these cells in early in vitro passages by anticomplement and Indirect Immunofluorescence tests. Infectious virus was rescued from one of the cell sublines after temperature-shock treatment at passage 26. Karyotypic analysis confirmed the human origin of the cells. Control uninfected kidney cancer cells survived only six in vitro passages. The established cells grew to more than 100 in vitro passages 1 year after Initiation of the experiments and induced an epithelioid cancer of variable morphology that Infiltrated nerves and muscles when Inoculated sc Into athymlc nude mice.

Original languageEnglish (US)
Pages (from-to)1313-1321
Number of pages9
JournalJournal of the National Cancer Institute
Volume63
Issue number6
DOIs
StatePublished - Jan 1 1979

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Epithelioid Cells
Herpesviridae
Cytomegalovirus
Kidney Neoplasms
Viruses
Cell Membrane
Nuclear Envelope
Indirect Fluorescent Antibody Technique
Nude Mice
Shock
Embryonic Structures
Cell Culture Techniques
Fibroblasts
In Vitro Techniques
Antigens
Cell Line
Muscles
Lung
Temperature
Infection

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Geder, Laszlo ; Ladda, Roger ; Kreider, John W. ; Sanford, Edgar J. ; Rapp, F. / Properties of human epithelioid cells established in vitro by a herpesvirus [ibrv(Hmc)] isolated from cytomegalovirus-transformed human cells. In: Journal of the National Cancer Institute. 1979 ; Vol. 63, No. 6. pp. 1313-1321.
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Properties of human epithelioid cells established in vitro by a herpesvirus [ibrv(Hmc)] isolated from cytomegalovirus-transformed human cells. / Geder, Laszlo; Ladda, Roger; Kreider, John W.; Sanford, Edgar J.; Rapp, F.

In: Journal of the National Cancer Institute, Vol. 63, No. 6, 01.01.1979, p. 1313-1321.

Research output: Contribution to journalArticle

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