Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration

Sang Mok Lee, Sujith Nair, Hye Kyung Ahn, Beom Soo Kim, Seung Hyun Jun, Hyo Jin An, Erik Hsiao, Seong Kim, Yoon Mo Koo, Jungbae Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Trypsin was coated onto electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers with varied concentrations of maleic anhydride group, which enables an easy conjugation of enzymes onto PS-PSMA nanofibers. The concentration of maleic anhydride group, determined by the amount of PSMA in the mixture of PS and PSMA for electrospinning, correlated well with the enzyme loading and activity of covalently attached trypsin. Trypsin-coated nanofibers, prepared via covalent attachment of seed enzyme molecules and follow-up enzyme cross-linking, resulted in highly stable nanobiocatalytic systems, irrespective of added PSMA amounts. The variation of PSMA amounts enables the facile control of various properties of trypsin-coated nanofibers, such as enzyme loading, activity, structure, and mass transfer limitation. Especially, the mass transfer limitation of substrate through the enzyme coating matrix correlated well with the conjugation site concentration, and this feature would be useful in optimizing the enzyme coatings for the biocatalytic conversion of large-size substrates, such as protein digestion as an example. Facile property control of highly stable enzyme coatings would provide a powerful tool for more successful applications of enzyme coatings in various fields.

Original languageEnglish (US)
Pages (from-to)216-221
Number of pages6
JournalEnzyme and Microbial Technology
Volume47
Issue number5
DOIs
StatePublished - Oct 1 2010

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Nanofibers
Polymers
Enzymes
Coatings
Maleic anhydride
Trypsin
Maleic Anhydrides
Polystyrenes
Styrene
Mass transfer
Electrospinning
Substrates
Proteolysis
Seed
Seeds
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Lee, Sang Mok ; Nair, Sujith ; Ahn, Hye Kyung ; Kim, Beom Soo ; Jun, Seung Hyun ; An, Hyo Jin ; Hsiao, Erik ; Kim, Seong ; Koo, Yoon Mo ; Kim, Jungbae. / Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration. In: Enzyme and Microbial Technology. 2010 ; Vol. 47, No. 5. pp. 216-221.
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Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration. / Lee, Sang Mok; Nair, Sujith; Ahn, Hye Kyung; Kim, Beom Soo; Jun, Seung Hyun; An, Hyo Jin; Hsiao, Erik; Kim, Seong; Koo, Yoon Mo; Kim, Jungbae.

In: Enzyme and Microbial Technology, Vol. 47, No. 5, 01.10.2010, p. 216-221.

Research output: Contribution to journalArticle

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T1 - Property control of enzyme coatings on polymer nanofibers by varying the conjugation site concentration

AU - Lee, Sang Mok

AU - Nair, Sujith

AU - Ahn, Hye Kyung

AU - Kim, Beom Soo

AU - Jun, Seung Hyun

AU - An, Hyo Jin

AU - Hsiao, Erik

AU - Kim, Seong

AU - Koo, Yoon Mo

AU - Kim, Jungbae

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