Prostaglandins mediate the endotoxin-induced suppression of pulsatile gonadotropin-releasing hormone and luteinizing hormone secretion in the ewe

Thomas G. Harris, Deborah F. Battaglia, Martha E. Brown, Morton B. Brown, Nichole E. Carlson, Catherine Viguié, Candace Y. Williams, Fred J. Karsch

Research output: Contribution to journalArticle

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Abstract

Five experiments were conducted to test the hypothesis that PGs mediate the endotoxin-induced inhibition of pulsatile GnRH and LH secretion in the ewe. Our approach was to test whether the PG synthesis inhibitor, flurbiprofen, could reverse the inhibitory effects of endotoxin on pulsatile LH and GnRH secretion in ovariectomized ewes. Exp 1-4 were cross-over experiments in which ewes received either flurbiprofen or vehicle 2 weeks apart. Jugular blood samples were taken for LH analysis throughout a 9-h experimental period. Depending on the specific purpose of the experiment, flurbiprofen or vehicle was administered after 3.5 h, followed by endotoxin, vehicle, or ovarian steroids (estradiol plus progesterone) at 4 h. In Exp 1, flurbiprofen reversed the endotoxin-induced suppression of mean serum LH concentrations and the elevation of body temperature, In Exp 2, flurbiprofen prevented the endotoxin-induced inhibition of pulsatile LH secretion and stimulation of fever, reduced the stimulation of plasma cortisol and progesterone, but did not affect the rise in circulating tumor necrosis factor-α. In Exp 3, flurbiprofen in the absence of endotoxin had no effect on pulsatile LH secretion. In Exp 4, flurbiprofen failed to prevent suppression of pulsatile LH secretion induced by luteal phase levels of the ovarian steroids progesterone and estradiol, which produce a nonimmune suppression of gonadotropin secretion. In Exp 5, flurbiprofen prevented the endotoxin-induced inhibition of pulsatile GnRH release into pituitary portal blood. Our finding that this PG synthesis inhibitor reverses the inhibitory effect of endotoxin leads to the conclusion that PGs mediate the suppressive effects of this immune/inflammatory challenge on pulsatile GnRH and LH secretion.

Original languageEnglish (US)
Pages (from-to)1050-1058
Number of pages9
JournalEndocrinology
Volume141
Issue number3
DOIs
StatePublished - Jan 1 2000

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Flurbiprofen
Luteinizing Hormone
Gonadotropin-Releasing Hormone
Endotoxins
Prostaglandins
Progesterone
Estradiol
Steroids
Luteal Phase
Body Temperature
Gonadotropins
Hydrocortisone
Fever
Neck
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Harris, T. G., Battaglia, D. F., Brown, M. E., Brown, M. B., Carlson, N. E., Viguié, C., ... Karsch, F. J. (2000). Prostaglandins mediate the endotoxin-induced suppression of pulsatile gonadotropin-releasing hormone and luteinizing hormone secretion in the ewe. Endocrinology, 141(3), 1050-1058. https://doi.org/10.1210/endo.141.3.7393
Harris, Thomas G. ; Battaglia, Deborah F. ; Brown, Martha E. ; Brown, Morton B. ; Carlson, Nichole E. ; Viguié, Catherine ; Williams, Candace Y. ; Karsch, Fred J. / Prostaglandins mediate the endotoxin-induced suppression of pulsatile gonadotropin-releasing hormone and luteinizing hormone secretion in the ewe. In: Endocrinology. 2000 ; Vol. 141, No. 3. pp. 1050-1058.
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abstract = "Five experiments were conducted to test the hypothesis that PGs mediate the endotoxin-induced inhibition of pulsatile GnRH and LH secretion in the ewe. Our approach was to test whether the PG synthesis inhibitor, flurbiprofen, could reverse the inhibitory effects of endotoxin on pulsatile LH and GnRH secretion in ovariectomized ewes. Exp 1-4 were cross-over experiments in which ewes received either flurbiprofen or vehicle 2 weeks apart. Jugular blood samples were taken for LH analysis throughout a 9-h experimental period. Depending on the specific purpose of the experiment, flurbiprofen or vehicle was administered after 3.5 h, followed by endotoxin, vehicle, or ovarian steroids (estradiol plus progesterone) at 4 h. In Exp 1, flurbiprofen reversed the endotoxin-induced suppression of mean serum LH concentrations and the elevation of body temperature, In Exp 2, flurbiprofen prevented the endotoxin-induced inhibition of pulsatile LH secretion and stimulation of fever, reduced the stimulation of plasma cortisol and progesterone, but did not affect the rise in circulating tumor necrosis factor-α. In Exp 3, flurbiprofen in the absence of endotoxin had no effect on pulsatile LH secretion. In Exp 4, flurbiprofen failed to prevent suppression of pulsatile LH secretion induced by luteal phase levels of the ovarian steroids progesterone and estradiol, which produce a nonimmune suppression of gonadotropin secretion. In Exp 5, flurbiprofen prevented the endotoxin-induced inhibition of pulsatile GnRH release into pituitary portal blood. Our finding that this PG synthesis inhibitor reverses the inhibitory effect of endotoxin leads to the conclusion that PGs mediate the suppressive effects of this immune/inflammatory challenge on pulsatile GnRH and LH secretion.",
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Harris, TG, Battaglia, DF, Brown, ME, Brown, MB, Carlson, NE, Viguié, C, Williams, CY & Karsch, FJ 2000, 'Prostaglandins mediate the endotoxin-induced suppression of pulsatile gonadotropin-releasing hormone and luteinizing hormone secretion in the ewe', Endocrinology, vol. 141, no. 3, pp. 1050-1058. https://doi.org/10.1210/endo.141.3.7393

Prostaglandins mediate the endotoxin-induced suppression of pulsatile gonadotropin-releasing hormone and luteinizing hormone secretion in the ewe. / Harris, Thomas G.; Battaglia, Deborah F.; Brown, Martha E.; Brown, Morton B.; Carlson, Nichole E.; Viguié, Catherine; Williams, Candace Y.; Karsch, Fred J.

In: Endocrinology, Vol. 141, No. 3, 01.01.2000, p. 1050-1058.

Research output: Contribution to journalArticle

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AU - Harris, Thomas G.

AU - Battaglia, Deborah F.

AU - Brown, Martha E.

AU - Brown, Morton B.

AU - Carlson, Nichole E.

AU - Viguié, Catherine

AU - Williams, Candace Y.

AU - Karsch, Fred J.

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