Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations

Role in vasculogenic mimicry

Navesh Sharma, Richard E.B. Seftor, Elisabeth A. Seftor, Lynn M. Gruman, Paul M. Heidger, Michael B. Cohen, David M. Lubaroff, Mary J.C. Hendrix

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

BACKGROUND. Tumor cell plasticity represents a significant clinical challenge in that the fate and function of tumor cells can be elusive until a tumor mass is evident. A remarkable example of plasticity is tumor cell vasculogenic mimicry, recently described in aggressive uveal and cutaneous melanoma, in addition to ovarian carcinoma, whereby tumor cells express endothelial-associated genes and form de novo vasculogenic-like networks in three-dimensional (3-D) culture. In the current investigation, we examined whether there is evidence for vasculogenic mimicry in heterogeneous prostatic neoplasms. METHODS. Dunning rat and human prostate cancer cell lines (comprised of epithelial- and fibroblastic-like tumor subpopulations) were tested for their ability to express selected endothelial-associated genes, laminin, the α6β1 laminin-binding integrin, and for their potential to form perfusable tubular networks in 3-D culture. Simultaneous morphological analysis of tumor-lined channels in rat and human tumors was also performed. RESULTS. Green fluorescent protein labeling of prostatic clonal subpopulations revealed unique cooperative interactions of epithelial- and fibroblastic-like tumor cells in the formation of perfusable vasculogenic-like networks. Furthermore, while these cell lines were shown to express various vascular markers, prostatic tumor cell-lined channels were also detected in vivo in high grade tumors, and occurred in some cases in close proximity to conventional endothelial-lined vasculature. CONCLUSIONS. A multidisciplinary approach to assess vasculogenic mimicry by prostatic tumor cells has revealed supportive evidence that it occurs in invasive, heterogeneous prostate cancer cell lines, and circumstantially in aggressive rat and human tumors. These results reflect the plasticity of aggressive prostatic tumor cells and may provide new prognostic markers for clinical diagnosis and new therapeutic intervention strategies. 189-201

Original languageEnglish (US)
Pages (from-to)189-201
Number of pages13
JournalProstate
Volume50
Issue number3
DOIs
StatePublished - Feb 15 2002

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Neoplasms
Prostatic Neoplasms
Laminin
Cell Line
Cell Plasticity
Tumor Biomarkers
Green Fluorescent Proteins
Integrins
Genes
Blood Vessels
Endothelial Cells
Biomarkers
Carcinoma
Skin

All Science Journal Classification (ASJC) codes

  • Oncology
  • Urology

Cite this

Sharma, N., Seftor, R. E. B., Seftor, E. A., Gruman, L. M., Heidger, P. M., Cohen, M. B., ... Hendrix, M. J. C. (2002). Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations: Role in vasculogenic mimicry. Prostate, 50(3), 189-201. https://doi.org/10.1002/pros.10048
Sharma, Navesh ; Seftor, Richard E.B. ; Seftor, Elisabeth A. ; Gruman, Lynn M. ; Heidger, Paul M. ; Cohen, Michael B. ; Lubaroff, David M. ; Hendrix, Mary J.C. / Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations : Role in vasculogenic mimicry. In: Prostate. 2002 ; Vol. 50, No. 3. pp. 189-201.
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Sharma, N, Seftor, REB, Seftor, EA, Gruman, LM, Heidger, PM, Cohen, MB, Lubaroff, DM & Hendrix, MJC 2002, 'Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations: Role in vasculogenic mimicry', Prostate, vol. 50, no. 3, pp. 189-201. https://doi.org/10.1002/pros.10048

Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations : Role in vasculogenic mimicry. / Sharma, Navesh; Seftor, Richard E.B.; Seftor, Elisabeth A.; Gruman, Lynn M.; Heidger, Paul M.; Cohen, Michael B.; Lubaroff, David M.; Hendrix, Mary J.C.

In: Prostate, Vol. 50, No. 3, 15.02.2002, p. 189-201.

Research output: Contribution to journalArticle

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T1 - Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations

T2 - Role in vasculogenic mimicry

AU - Sharma, Navesh

AU - Seftor, Richard E.B.

AU - Seftor, Elisabeth A.

AU - Gruman, Lynn M.

AU - Heidger, Paul M.

AU - Cohen, Michael B.

AU - Lubaroff, David M.

AU - Hendrix, Mary J.C.

PY - 2002/2/15

Y1 - 2002/2/15

N2 - BACKGROUND. Tumor cell plasticity represents a significant clinical challenge in that the fate and function of tumor cells can be elusive until a tumor mass is evident. A remarkable example of plasticity is tumor cell vasculogenic mimicry, recently described in aggressive uveal and cutaneous melanoma, in addition to ovarian carcinoma, whereby tumor cells express endothelial-associated genes and form de novo vasculogenic-like networks in three-dimensional (3-D) culture. In the current investigation, we examined whether there is evidence for vasculogenic mimicry in heterogeneous prostatic neoplasms. METHODS. Dunning rat and human prostate cancer cell lines (comprised of epithelial- and fibroblastic-like tumor subpopulations) were tested for their ability to express selected endothelial-associated genes, laminin, the α6β1 laminin-binding integrin, and for their potential to form perfusable tubular networks in 3-D culture. Simultaneous morphological analysis of tumor-lined channels in rat and human tumors was also performed. RESULTS. Green fluorescent protein labeling of prostatic clonal subpopulations revealed unique cooperative interactions of epithelial- and fibroblastic-like tumor cells in the formation of perfusable vasculogenic-like networks. Furthermore, while these cell lines were shown to express various vascular markers, prostatic tumor cell-lined channels were also detected in vivo in high grade tumors, and occurred in some cases in close proximity to conventional endothelial-lined vasculature. CONCLUSIONS. A multidisciplinary approach to assess vasculogenic mimicry by prostatic tumor cells has revealed supportive evidence that it occurs in invasive, heterogeneous prostate cancer cell lines, and circumstantially in aggressive rat and human tumors. These results reflect the plasticity of aggressive prostatic tumor cells and may provide new prognostic markers for clinical diagnosis and new therapeutic intervention strategies. 189-201

AB - BACKGROUND. Tumor cell plasticity represents a significant clinical challenge in that the fate and function of tumor cells can be elusive until a tumor mass is evident. A remarkable example of plasticity is tumor cell vasculogenic mimicry, recently described in aggressive uveal and cutaneous melanoma, in addition to ovarian carcinoma, whereby tumor cells express endothelial-associated genes and form de novo vasculogenic-like networks in three-dimensional (3-D) culture. In the current investigation, we examined whether there is evidence for vasculogenic mimicry in heterogeneous prostatic neoplasms. METHODS. Dunning rat and human prostate cancer cell lines (comprised of epithelial- and fibroblastic-like tumor subpopulations) were tested for their ability to express selected endothelial-associated genes, laminin, the α6β1 laminin-binding integrin, and for their potential to form perfusable tubular networks in 3-D culture. Simultaneous morphological analysis of tumor-lined channels in rat and human tumors was also performed. RESULTS. Green fluorescent protein labeling of prostatic clonal subpopulations revealed unique cooperative interactions of epithelial- and fibroblastic-like tumor cells in the formation of perfusable vasculogenic-like networks. Furthermore, while these cell lines were shown to express various vascular markers, prostatic tumor cell-lined channels were also detected in vivo in high grade tumors, and occurred in some cases in close proximity to conventional endothelial-lined vasculature. CONCLUSIONS. A multidisciplinary approach to assess vasculogenic mimicry by prostatic tumor cells has revealed supportive evidence that it occurs in invasive, heterogeneous prostate cancer cell lines, and circumstantially in aggressive rat and human tumors. These results reflect the plasticity of aggressive prostatic tumor cells and may provide new prognostic markers for clinical diagnosis and new therapeutic intervention strategies. 189-201

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