Protargol silver impregnation and neuronal selectivity. Light and electron microscopic observations

Ian Zagon, J. H. Haring

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The neuronal selectivity of protargol silver was determined in embedded and cultured nervous tissue by light (LM) and transmission electron microscopy (TEM). Ultrastructurally, silver (85-140 Å in diameter) was found deposited in neuronal, but not glial, perikarya and cell processes. Usually, the silver grains were arranged in elongated arrays that were associated with electron-dense, strand-like structures that correspond to the 'neurofibrils' observed in LM; myelin sheaths, mitochondria, Nissl substance, Golgi bodies, synaptic vesicles, and endoplasmic reticulum could be excluded as the basis for neuronal argyrophila. Unfortunately, the composition of these neurofibrils was obscure because of poor tissue preservation involved with proper impregnation, but they appeared to correspond to microtubules and neurofilaments. Neuronal structures containing a predominance of either organelle were impregnated with protargol. Nuclei and nucleoli of neuronal and nonneuronal cells were densely stained with silver as visualized with LM and TEM. Not every neuron was impregnated with protargol, but under conditions of improper fixation some of these neurons had an electron-dense cytoplasm which exhibited a definite affinity for silver. A discussion of protargol selectivity is also presented.

Original languageEnglish (US)
Pages (from-to)193-210
Number of pages18
JournalActa Anatomica
Volume114
Issue number3
StatePublished - Dec 1 1982

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Silver
Electrons
Neurofibrils
Light
Transmission Electron Microscopy
Tissue Preservation
Neurons
Nerve Tissue
Intermediate Filaments
Synaptic Vesicles
Myelin Sheath
Microtubules
Neuroglia
Endoplasmic Reticulum
Organelles
Mitochondria
Cytoplasm
strong silver protein

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

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abstract = "The neuronal selectivity of protargol silver was determined in embedded and cultured nervous tissue by light (LM) and transmission electron microscopy (TEM). Ultrastructurally, silver (85-140 {\AA} in diameter) was found deposited in neuronal, but not glial, perikarya and cell processes. Usually, the silver grains were arranged in elongated arrays that were associated with electron-dense, strand-like structures that correspond to the 'neurofibrils' observed in LM; myelin sheaths, mitochondria, Nissl substance, Golgi bodies, synaptic vesicles, and endoplasmic reticulum could be excluded as the basis for neuronal argyrophila. Unfortunately, the composition of these neurofibrils was obscure because of poor tissue preservation involved with proper impregnation, but they appeared to correspond to microtubules and neurofilaments. Neuronal structures containing a predominance of either organelle were impregnated with protargol. Nuclei and nucleoli of neuronal and nonneuronal cells were densely stained with silver as visualized with LM and TEM. Not every neuron was impregnated with protargol, but under conditions of improper fixation some of these neurons had an electron-dense cytoplasm which exhibited a definite affinity for silver. A discussion of protargol selectivity is also presented.",
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Protargol silver impregnation and neuronal selectivity. Light and electron microscopic observations. / Zagon, Ian; Haring, J. H.

In: Acta Anatomica, Vol. 114, No. 3, 01.12.1982, p. 193-210.

Research output: Contribution to journalArticle

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