Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites

Stephen L. Hoffman, Lucy M.L. Goh, Thomas C. Luke, Imogene Schneider, Thong P. Le, Denise L. Doolan, John Sacci, Patricia De la Vega, Megan Dowler, Chris Paul, Daniel M. Gordon, Jose A. Stoute, L. W. Preston Church, Martha Sedegah, D. Gray Heppner, W. Ripley Ballou, Thomas L. Richie

Research output: Contribution to journalArticle

523 Citations (Scopus)

Abstract

During 1989-1999, 11 volunteers were immunized by the bites of 1001-2927 irradiated mosquitoes harboring infectious sporozoites of Plasmodium falciparum (Pf) strain NF54 or clone 3D7/NF54. Ten volunteers were first challenged by the bites of Pf-infected mosquitoes 2-9 weeks after the last immunization, and all were protected. A volunteer challenged 10 weeks after the last immunization was not protected. Five previously protected volunteers were rechallenged 23-42 weeks after a secondary immunization, and 4 were protected. Two volunteers were protected when rechallenged with a heterologous Pf strain (7G8). In total, there was protection in 24 of 26 challenges. These results expand published findings demonstrating that immunization by exposure to thousands of mosquitoes carrying radiation-attenuated Pf sporozoites is safe and well tolerated and elicits strain-transcendent protective immunity that persists for at least 42 weeks.

Original languageEnglish (US)
Pages (from-to)1155-1164
Number of pages10
JournalJournal of Infectious Diseases
Volume185
Issue number8
DOIs
StatePublished - Apr 15 2002

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Sporozoites
Plasmodium falciparum
Malaria
Volunteers
Immunization
Radiation
Culicidae
Bites and Stings
Secondary Immunization
Immunity
Clone Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Hoffman, S. L., Goh, L. M. L., Luke, T. C., Schneider, I., Le, T. P., Doolan, D. L., ... Richie, T. L. (2002). Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites. Journal of Infectious Diseases, 185(8), 1155-1164. https://doi.org/10.1086/339409
Hoffman, Stephen L. ; Goh, Lucy M.L. ; Luke, Thomas C. ; Schneider, Imogene ; Le, Thong P. ; Doolan, Denise L. ; Sacci, John ; De la Vega, Patricia ; Dowler, Megan ; Paul, Chris ; Gordon, Daniel M. ; Stoute, Jose A. ; Preston Church, L. W. ; Sedegah, Martha ; Gray Heppner, D. ; Ripley Ballou, W. ; Richie, Thomas L. / Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites. In: Journal of Infectious Diseases. 2002 ; Vol. 185, No. 8. pp. 1155-1164.
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abstract = "During 1989-1999, 11 volunteers were immunized by the bites of 1001-2927 irradiated mosquitoes harboring infectious sporozoites of Plasmodium falciparum (Pf) strain NF54 or clone 3D7/NF54. Ten volunteers were first challenged by the bites of Pf-infected mosquitoes 2-9 weeks after the last immunization, and all were protected. A volunteer challenged 10 weeks after the last immunization was not protected. Five previously protected volunteers were rechallenged 23-42 weeks after a secondary immunization, and 4 were protected. Two volunteers were protected when rechallenged with a heterologous Pf strain (7G8). In total, there was protection in 24 of 26 challenges. These results expand published findings demonstrating that immunization by exposure to thousands of mosquitoes carrying radiation-attenuated Pf sporozoites is safe and well tolerated and elicits strain-transcendent protective immunity that persists for at least 42 weeks.",
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Hoffman, SL, Goh, LML, Luke, TC, Schneider, I, Le, TP, Doolan, DL, Sacci, J, De la Vega, P, Dowler, M, Paul, C, Gordon, DM, Stoute, JA, Preston Church, LW, Sedegah, M, Gray Heppner, D, Ripley Ballou, W & Richie, TL 2002, 'Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites', Journal of Infectious Diseases, vol. 185, no. 8, pp. 1155-1164. https://doi.org/10.1086/339409

Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites. / Hoffman, Stephen L.; Goh, Lucy M.L.; Luke, Thomas C.; Schneider, Imogene; Le, Thong P.; Doolan, Denise L.; Sacci, John; De la Vega, Patricia; Dowler, Megan; Paul, Chris; Gordon, Daniel M.; Stoute, Jose A.; Preston Church, L. W.; Sedegah, Martha; Gray Heppner, D.; Ripley Ballou, W.; Richie, Thomas L.

In: Journal of Infectious Diseases, Vol. 185, No. 8, 15.04.2002, p. 1155-1164.

Research output: Contribution to journalArticle

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T1 - Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites

AU - Hoffman, Stephen L.

AU - Goh, Lucy M.L.

AU - Luke, Thomas C.

AU - Schneider, Imogene

AU - Le, Thong P.

AU - Doolan, Denise L.

AU - Sacci, John

AU - De la Vega, Patricia

AU - Dowler, Megan

AU - Paul, Chris

AU - Gordon, Daniel M.

AU - Stoute, Jose A.

AU - Preston Church, L. W.

AU - Sedegah, Martha

AU - Gray Heppner, D.

AU - Ripley Ballou, W.

AU - Richie, Thomas L.

PY - 2002/4/15

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N2 - During 1989-1999, 11 volunteers were immunized by the bites of 1001-2927 irradiated mosquitoes harboring infectious sporozoites of Plasmodium falciparum (Pf) strain NF54 or clone 3D7/NF54. Ten volunteers were first challenged by the bites of Pf-infected mosquitoes 2-9 weeks after the last immunization, and all were protected. A volunteer challenged 10 weeks after the last immunization was not protected. Five previously protected volunteers were rechallenged 23-42 weeks after a secondary immunization, and 4 were protected. Two volunteers were protected when rechallenged with a heterologous Pf strain (7G8). In total, there was protection in 24 of 26 challenges. These results expand published findings demonstrating that immunization by exposure to thousands of mosquitoes carrying radiation-attenuated Pf sporozoites is safe and well tolerated and elicits strain-transcendent protective immunity that persists for at least 42 weeks.

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