Protection of rabbits from viral challenge by gene gun-based intracutaneous vaccination with a combination of cottontail rabbit papillomavirus E1, E2, E6, and E7 genes

Ricai Han, Nancy M. Cladel, Cynthia A. Reed, Xuwen Peng, Neil Christensen

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In this study, cottontail rabbit papillomavirus infection of domestic rabbits was used as an animal model to develop papillomavirus early gene- based vaccines. Groups of rabbits were intracutaneously vaccinated with single papillomavirus early genes E1, E2, E6, and E7 or with a combination of these four genes. Only a fraction of rabbits were protected from subsequent viral challenge when vaccinated with the E1 or E6 gene. Viral tumor growth in those rabbits vaccinated with the E1 or E2 gene was suppressed compared to that in controls. In contrast, seven of nine rabbits vaccinated with the combination of the E1, E2, E6, and E7 genes were completely protected against viral challenge. These data indicated that intracutaneous genetic vaccination with the combination of the E1, E2, E6, and E7 genes can be an effective strategy for immunoprophylaxis of papillomavirus infection.

Original languageEnglish (US)
Pages (from-to)7039-7043
Number of pages5
JournalJournal of virology
Volume73
Issue number8
StatePublished - Jan 1 1999

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Cottontail rabbit papillomavirus
Viral Genes
Firearms
Vaccination
rabbits
vaccination
Rabbits
Papillomaviridae
Genes
genes
Papillomavirus Infections
infection
Vaccines
Animal Models
animal models
vaccines

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "In this study, cottontail rabbit papillomavirus infection of domestic rabbits was used as an animal model to develop papillomavirus early gene- based vaccines. Groups of rabbits were intracutaneously vaccinated with single papillomavirus early genes E1, E2, E6, and E7 or with a combination of these four genes. Only a fraction of rabbits were protected from subsequent viral challenge when vaccinated with the E1 or E6 gene. Viral tumor growth in those rabbits vaccinated with the E1 or E2 gene was suppressed compared to that in controls. In contrast, seven of nine rabbits vaccinated with the combination of the E1, E2, E6, and E7 genes were completely protected against viral challenge. These data indicated that intracutaneous genetic vaccination with the combination of the E1, E2, E6, and E7 genes can be an effective strategy for immunoprophylaxis of papillomavirus infection.",
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Protection of rabbits from viral challenge by gene gun-based intracutaneous vaccination with a combination of cottontail rabbit papillomavirus E1, E2, E6, and E7 genes. / Han, Ricai; Cladel, Nancy M.; Reed, Cynthia A.; Peng, Xuwen; Christensen, Neil.

In: Journal of virology, Vol. 73, No. 8, 01.01.1999, p. 7039-7043.

Research output: Contribution to journalArticle

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