Protection of susceptible BALB/c mice from challenge with Leishmania major by nucleoside hydrolase, a soluble exo-antigen of Leishmania

Mohammad A. Al-Wabel, Willy K. Tonui, Liwang Cui, Samuel K. Martin, Richard G. Titus

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Leishmania major culture-derived, soluble, exogenous antigens have been shown to be a source of vaccine targets for the parasite. We have previously reported that L. major culture-derived, soluble, exogenous antigens can immunize BALB/c mice against challenge with L. major. However, the molecule(s) involved in this protection was not known. We describe the potential of one component of soluble exogenous antigens (recombinant nucleoside hydrolase) to vaccinate mice against challenge with L. major. We found that recombinant nucleoside hydrolase vaccinated BALB/c mice against a subsequent challenge with L. major. Protection was manifested by a significant decrease in lesion size (as much as a 30-fold reduction) and parasite burden (as much as a 71-fold reduction). Protection was achieved whether recombinant nucleoside hydrolase was administered to mice in the presence or absence of adjuvant (interleukin-12) Finally, protection was accompanied by an increase in interferon-γ production but a decrease in interleukin-10 production by vaccinated animals in response to challenge with L. major.

Original languageEnglish (US)
Pages (from-to)1060-1065
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number6
DOIs
StatePublished - Dec 2007

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Virology
  • Infectious Diseases

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