Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila

P. E. Jablonski, A. A. DiMarco, T. A. Bobik, M. C. Cabell, J. G. Ferry

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The cell extract protein content of acetate- and methanol-grown Methanosarcina thermophila TM-1 was examined by two-dimensional polyacrylamide gel electrophoresis. More than 100 mutually exclusive spots were present in acetate- and methanol-grown cells. Spots corresponding to acetate kinase, phosphotransacetylase, and the five subunits of the carbon monoxide dehydrogenase complex were identified in acetate-grown cells. Activities of formylmethanofuran dehydrogenase, formylmethanofuran:tetrahydromethanopterin formyltransferase, 5,10-methenyltetrahydromethanopterin cyclohydrolase, methylene tetrahydromethanopterin:coenzyme F420 oxidoreductase, formate dehydrogenase, and carbonic anhydrase were examined in acetate- and methanol-grown Methanosarcina thermophila. Levels of formyltransferase in either acetate- or methanol-grown Methanosarcina thermophila were approximately half the levels detected in H2-CO2-grown Methanobacterium thermoautotrophicum. All other enzyme activities were significantly lower in acetate- and methanol-grown Methanosarcina thermophila.

Original languageEnglish (US)
Pages (from-to)1271-1275
Number of pages5
JournalJournal of bacteriology
Volume172
Issue number3
DOIs
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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