Protein Disulfide Isomerase-Like Protein 1-1 Controls Endosperm Development through Regulation of the Amount and Composition of Seed Proteins in Rice

Yeon Jeong Kim, Song Yion Yeu, Bongsoo Park, Hee Jong Koh, Jong Tae Song, Hak Soo Seo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Protein disulfide isomerase (PDI) is a chaperone protein involved in oxidative protein folding by acting as a catalyst and assisting folding in the endoplasmic reticulum (ER). A genome database search showed that rice contains 19 PDI-like genes. However, their functions are not clearly identified. This paper shows possible functions of rice PDI-like protein 1-1 (PDIL1-1) during seed development. Seeds of the T-DNA insertion PDIL1-1 mutant, PDIL1-1Δ, identified by genomic DNA PCR and western blot analysis, display a chalky phenotype and a thick aleurone layer. Protein content per seed was significantly lower and free sugar content higher in PDIL1-1Δ mutant seeds than in the wild type. Proteomic analysis of PDIL1-1Δ mutant seeds showed that PDIL1-1 is post-translationally regulated, and its loss causes accumulation of many types of seed proteins including glucose/starch metabolism- and ROS (reactive oxygen species) scavenging-related proteins. In addition, PDIL1-1 strongly interacts with the cysteine protease OsCP1. Our data indicate that the opaque phenotype of PDIL1-1Δ mutant seeds results from production of irregular starch granules and protein body through loss of regulatory activity for various proteins involved in the synthesis of seed components.

Original languageEnglish (US)
Article numbere44493
JournalPloS one
Volume7
Issue number9
DOIs
StatePublished - Sep 6 2012

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protein disulfide-isomerase
Protein Disulfide-Isomerases
rice protein
Endosperm
endosperm
Seed
Seeds
seeds
Chemical analysis
Proteins
proteins
mutants
Oryza
Starch
phenotype
aleurone layer
Genes
protein bodies
protein folding
cysteine proteinases

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Kim, Yeon Jeong ; Yeu, Song Yion ; Park, Bongsoo ; Koh, Hee Jong ; Song, Jong Tae ; Seo, Hak Soo. / Protein Disulfide Isomerase-Like Protein 1-1 Controls Endosperm Development through Regulation of the Amount and Composition of Seed Proteins in Rice. In: PloS one. 2012 ; Vol. 7, No. 9.
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Protein Disulfide Isomerase-Like Protein 1-1 Controls Endosperm Development through Regulation of the Amount and Composition of Seed Proteins in Rice. / Kim, Yeon Jeong; Yeu, Song Yion; Park, Bongsoo; Koh, Hee Jong; Song, Jong Tae; Seo, Hak Soo.

In: PloS one, Vol. 7, No. 9, e44493, 06.09.2012.

Research output: Contribution to journalArticle

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