Protein glutathiolation in human blood

Wayne A. Kleinman, Despina Komninou, Yvonne Leutzinger, Stephen Colosimo, Julie Cox, Calvin A. Lang, John P. Richie

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Abstract

Glutathione (GSH) exists in both free and protein-bound (glutathiolated) forms (GSSP). Protein glutathiolation may represent an important post-translational regulatory mechanism for proteins. However, there are little data regarding the regulation of glutathiolation in blood. Our objectives were to examine GSSP levels of human blood by determining the distribution and variability of blood GSSP, as well as its relationship to free GSH and hemoglobin in healthy adults. To this end, we used a newly modified method allowing for rapid analysis of both GSH and GSSP in blood. GSSP was found in red cells with levels ranging from 4 to 27% of total (free+bound) GSH (mean±SD: 12.1±4.5%) with a concentration of 0.13±0.05μEq GSH/mL (mean±SD). No correlations were observed between GSSP and either GSH (r=-0.085) or hemoglobin (r=0.086). Together these results suggest that the extent of protein glutathiolation in blood is substantial (∼0.1mmol/L). While the interindividual variation in GSSP is large (34%), its levels are apparently not regulated by GSH content.

Original languageEnglish (US)
Pages (from-to)741-746
Number of pages6
JournalBiochemical Pharmacology
Volume65
Issue number5
DOIs
StatePublished - Mar 1 2003

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Blood
Proteins
Hemoglobins
Glutathione
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

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Kleinman, W. A., Komninou, D., Leutzinger, Y., Colosimo, S., Cox, J., Lang, C. A., & Richie, J. P. (2003). Protein glutathiolation in human blood. Biochemical Pharmacology, 65(5), 741-746. https://doi.org/10.1016/S0006-2952(02)01560-5
Kleinman, Wayne A. ; Komninou, Despina ; Leutzinger, Yvonne ; Colosimo, Stephen ; Cox, Julie ; Lang, Calvin A. ; Richie, John P. / Protein glutathiolation in human blood. In: Biochemical Pharmacology. 2003 ; Vol. 65, No. 5. pp. 741-746.
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Kleinman, WA, Komninou, D, Leutzinger, Y, Colosimo, S, Cox, J, Lang, CA & Richie, JP 2003, 'Protein glutathiolation in human blood', Biochemical Pharmacology, vol. 65, no. 5, pp. 741-746. https://doi.org/10.1016/S0006-2952(02)01560-5

Protein glutathiolation in human blood. / Kleinman, Wayne A.; Komninou, Despina; Leutzinger, Yvonne; Colosimo, Stephen; Cox, Julie; Lang, Calvin A.; Richie, John P.

In: Biochemical Pharmacology, Vol. 65, No. 5, 01.03.2003, p. 741-746.

Research output: Contribution to journalArticle

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Kleinman WA, Komninou D, Leutzinger Y, Colosimo S, Cox J, Lang CA et al. Protein glutathiolation in human blood. Biochemical Pharmacology. 2003 Mar 1;65(5):741-746. https://doi.org/10.1016/S0006-2952(02)01560-5