Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae

Tomohiro Yorimitsu, Shadia Zaman, James Broach, Daniel J. Klionsky

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Autophagy is a highly conserved, degradative process in eukaryotic cells. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that the protein kinase A (PKA) and Sch9 signaling pathways regulate autophagy cooperatively in yeast. Autophagy is induced in cells when PKA and Sch9 are simultaneously inactivated. Mutant alleles of these kinases bearing a mutation that confers sensitivity to the ATP-analogue inhibitor C3-1′-naphthyl-methyl PP1 revealed that autophagy was induced independently of effects on Tor kinase. The PKA-Sch9-mediated autophagy depends on the autophagy-related 1 kinase complex, which is also essential for TORC1-regulated autophagy, the transcription factors Msn2/4, and the Rim15 kinase. The present results suggest that autophagy is controlled by the signals from at least three partly separate nutrient-sensing pathways that include PKA, Sch9, and TORC1.

Original languageEnglish (US)
Pages (from-to)4180-4189
Number of pages10
JournalMolecular biology of the cell
Volume18
Issue number10
DOIs
StatePublished - Oct 1 2007

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Autophagy
Cyclic AMP-Dependent Protein Kinases
Saccharomyces cerevisiae
Phosphotransferases
Eukaryotic Cells
Sirolimus
Transcription Factors
Adenosine Triphosphate
Yeasts
Alleles
Food
Mutation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yorimitsu, Tomohiro ; Zaman, Shadia ; Broach, James ; Klionsky, Daniel J. / Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae. In: Molecular biology of the cell. 2007 ; Vol. 18, No. 10. pp. 4180-4189.
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Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae. / Yorimitsu, Tomohiro; Zaman, Shadia; Broach, James; Klionsky, Daniel J.

In: Molecular biology of the cell, Vol. 18, No. 10, 01.10.2007, p. 4180-4189.

Research output: Contribution to journalArticle

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