Protein localization and dynamics within a bacterial organelle

H. Velocity Hughes, Edgar Huitema, Sean Pritchard, Kenneth C. Keiler, Yves V. Brun, Patrick H. Viollier

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Protein localization mechanisms dictate the functional and structural specialization of cells. Of the four polar surface organelles featured by the dimorphic bacterium Caulobacter crescentus, the stalk, a cylindrical extension of all cell envelope layers, is the least well characterized at the molecular level. Here we apply a powerful experimental scheme that integrates genetics with high-throughput localization to discover StpX, an uncharacterized bitopic membrane protein that modulates stalk elongation and is sequestered to the stalk. In stalkless mutants StpX is dispersed. Two populations of StpX were discernible within the stalk with different mobilities: an immobile one near the stalk base and a mobile one near the stalk tip. Molecular anatomy provides evidence that (i) the StpX transmembrane domain enables access to the stalk organelle, (ii) the N-terminal periplasmic domain mediates retention in the stalk, and (iii) the C-terminal cytoplasmic domain enhances diffusion within the stalk. Moreover, the accumulation of StpX and an N-terminally truncated isoformis differentially coordinated with the cell cycle. Thus, at the submicron scale the localization and the mobility of a protein are precisely regulated in space and time and are important for the correct organization of a subcellular compartment or organelle such as the stalk.

Original languageEnglish (US)
Pages (from-to)5599-5604
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number12
DOIs
StatePublished - Mar 23 2010

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Organelles
Caulobacter crescentus
Proteins
Anatomy
Cell Cycle
Membrane Proteins
Bacteria
Population

All Science Journal Classification (ASJC) codes

  • General

Cite this

Hughes, H. Velocity ; Huitema, Edgar ; Pritchard, Sean ; Keiler, Kenneth C. ; Brun, Yves V. ; Viollier, Patrick H. / Protein localization and dynamics within a bacterial organelle. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 12. pp. 5599-5604.
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Protein localization and dynamics within a bacterial organelle. / Hughes, H. Velocity; Huitema, Edgar; Pritchard, Sean; Keiler, Kenneth C.; Brun, Yves V.; Viollier, Patrick H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 12, 23.03.2010, p. 5599-5604.

Research output: Contribution to journalArticle

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