Protein-protein and protein-DNA interactions of σ70 region 4 involved in transcription activation by λcI

Bryce E. Nickels, Simon L. Dove, Katsuhiko S. Murakami, Seth A. Darst, Ann Hochschild

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

The cI protein of bacteriophage λ (λcI) activates transcription from promoter PRM through an acidic patch on the surface of its DNA-binding domain. Genetic evidence suggests that this acidic patch stimulates transcription from PRM through contact with the C-terminal domain (region 4) of the σ70 subunit of Escherichia coli RNA polymerase. Here, we identify two basic residues in region 4 of σ70 that are critical for λcI-mediated activation of transcription from PRM. On the basis of structural modeling, we propose that one of these σ70 residues, K593, facilitates the interaction between λcI and region 4 of σ70 by inducing a bend in the DNA upstream of the -35 element, whereas the other, R588, interacts directly with a critical acidic residue within the activating patch of λcI. Residue R588 of σ70 has been shown to play an important role in promoter recognition; our findings suggest that the R588 side-chain has a dual function at PRM, facilitating the interaction of region 4 with the promoter -35 element and participating directly in the protein-protein interaction with λcI.

Original languageEnglish (US)
Pages (from-to)17-34
Number of pages18
JournalJournal of Molecular Biology
Volume324
Issue number1
DOIs
StatePublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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