Protein synthesis in rabbit reticulocytes

Characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates

R. O. Ralston, A. Das, A. Dasgupta, Reena Roy, S. Palmieri, N. K. Gupta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A ribosomal salt (0.5 M KCl) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNA(f)(Met)-binding factor (EIF-1), Co-EIF-1, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No direct correlation exists between amount of EIF-1 activity and ability to reverse inhibition of protein synthesis in heme-deficient lysates. Homogeneous preparations of EIF-1 are completely inactive in reversal of protein synthesis inhibition in heme-deficient lysates. These findings suggest that RF activity is not due to EIF-1 alone but may or may not require EIF-1 as a component of a complex factor.

Original languageEnglish (US)
Pages (from-to)4858-4862
Number of pages5
JournalUnknown Journal
Volume75
Issue number10
DOIs
StatePublished - Jan 1 1978

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Eukaryotic Initiation Factor-1
Reticulocytes
Heme
Rabbits
Proteins
Ternary Complex Factors
Eukaryotic Initiation Factor-2
Hemin
Salts

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Protein synthesis in rabbit reticulocytes: Characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates",
abstract = "A ribosomal salt (0.5 M KCl) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNA(f)(Met)-binding factor (EIF-1), Co-EIF-1, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No direct correlation exists between amount of EIF-1 activity and ability to reverse inhibition of protein synthesis in heme-deficient lysates. Homogeneous preparations of EIF-1 are completely inactive in reversal of protein synthesis inhibition in heme-deficient lysates. These findings suggest that RF activity is not due to EIF-1 alone but may or may not require EIF-1 as a component of a complex factor.",
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Protein synthesis in rabbit reticulocytes : Characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates. / Ralston, R. O.; Das, A.; Dasgupta, A.; Roy, Reena; Palmieri, S.; Gupta, N. K.

In: Unknown Journal, Vol. 75, No. 10, 01.01.1978, p. 4858-4862.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein synthesis in rabbit reticulocytes

T2 - Characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates

AU - Ralston, R. O.

AU - Das, A.

AU - Dasgupta, A.

AU - Roy, Reena

AU - Palmieri, S.

AU - Gupta, N. K.

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