TY - JOUR
T1 - Protein synthesis in rabbit reticulocytes
T2 - Characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates
AU - Ralston, R. O.
AU - Das, A.
AU - Dasgupta, A.
AU - Roy, R.
AU - Palmieri, S.
AU - Gupta, N. K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1978
Y1 - 1978
N2 - A ribosomal salt (0.5 M KCl) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNA(f)(Met)-binding factor (EIF-1), Co-EIF-1, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No direct correlation exists between amount of EIF-1 activity and ability to reverse inhibition of protein synthesis in heme-deficient lysates. Homogeneous preparations of EIF-1 are completely inactive in reversal of protein synthesis inhibition in heme-deficient lysates. These findings suggest that RF activity is not due to EIF-1 alone but may or may not require EIF-1 as a component of a complex factor.
AB - A ribosomal salt (0.5 M KCl) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNA(f)(Met)-binding factor (EIF-1), Co-EIF-1, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No direct correlation exists between amount of EIF-1 activity and ability to reverse inhibition of protein synthesis in heme-deficient lysates. Homogeneous preparations of EIF-1 are completely inactive in reversal of protein synthesis inhibition in heme-deficient lysates. These findings suggest that RF activity is not due to EIF-1 alone but may or may not require EIF-1 as a component of a complex factor.
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U2 - 10.1073/pnas.75.10.4858
DO - 10.1073/pnas.75.10.4858
M3 - Article
C2 - 283396
AN - SCOPUS:0018119546
VL - 75
SP - 4858
EP - 4862
JO - [No source information available]
JF - [No source information available]
SN - 0042-1215
IS - 10
ER -