Protein synthesis in rabbit reticulocytes

Characteristics of Co-eIF-2 protein complex

Milan K. Bagchi, Ambica C. Banerjee, Reena Roy, Indrani Chakrabarty, Naba K. Gupta

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNA, binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatment, and thus appears to be due to the combined action of both heat and NEM-insensitive Co-eIF-2A, and heat and NEM-sensitive Co-eIF-2C activities. (3) [3H]GDP bound in eIF-2·[3H]GDP complex is rapidly displaced by unlabelled GTP during ternary complex formation., Co-eIF-2 stimulates. Met-tRNAf binding to eIF-2 even when added after the [3H]GDP from eIF-2·[3H]GDP has been completely displaced. This indicates that Co-eIF-2-stimulation is not due to GDP displacement from eIF-2·GDP. We propose that eIF-2 molecules become inactive in the presence of Mg2 and at high dilution, and Co-eIF-2 restores the inactive eIF-2 molecules into an active form.

Original languageEnglish (US)
Pages (from-to)6501-6510
Number of pages10
JournalNucleic acids research
Volume10
Issue number20
DOIs
StatePublished - Oct 25 1982

Fingerprint

Reticulocytes
Hot Temperature
Rabbits
RNA, Transfer, Met
Proteins
Guanosine Triphosphate
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Bagchi, Milan K. ; Banerjee, Ambica C. ; Roy, Reena ; Chakrabarty, Indrani ; Gupta, Naba K. / Protein synthesis in rabbit reticulocytes : Characteristics of Co-eIF-2 protein complex. In: Nucleic acids research. 1982 ; Vol. 10, No. 20. pp. 6501-6510.
@article{62896a9fed86426380b7efe5dd2e2f77,
title = "Protein synthesis in rabbit reticulocytes: Characteristics of Co-eIF-2 protein complex",
abstract = "A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNA, binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatment, and thus appears to be due to the combined action of both heat and NEM-insensitive Co-eIF-2A, and heat and NEM-sensitive Co-eIF-2C activities. (3) [3H]GDP bound in eIF-2·[3H]GDP complex is rapidly displaced by unlabelled GTP during ternary complex formation., Co-eIF-2 stimulates. Met-tRNAf binding to eIF-2 even when added after the [3H]GDP from eIF-2·[3H]GDP has been completely displaced. This indicates that Co-eIF-2-stimulation is not due to GDP displacement from eIF-2·GDP. We propose that eIF-2 molecules become inactive in the presence of Mg2 and at high dilution, and Co-eIF-2 restores the inactive eIF-2 molecules into an active form.",
author = "Bagchi, {Milan K.} and Banerjee, {Ambica C.} and Reena Roy and Indrani Chakrabarty and Gupta, {Naba K.}",
year = "1982",
month = "10",
day = "25",
doi = "10.1093/nar/10.20.6501",
language = "English (US)",
volume = "10",
pages = "6501--6510",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "20",

}

Protein synthesis in rabbit reticulocytes : Characteristics of Co-eIF-2 protein complex. / Bagchi, Milan K.; Banerjee, Ambica C.; Roy, Reena; Chakrabarty, Indrani; Gupta, Naba K.

In: Nucleic acids research, Vol. 10, No. 20, 25.10.1982, p. 6501-6510.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein synthesis in rabbit reticulocytes

T2 - Characteristics of Co-eIF-2 protein complex

AU - Bagchi, Milan K.

AU - Banerjee, Ambica C.

AU - Roy, Reena

AU - Chakrabarty, Indrani

AU - Gupta, Naba K.

PY - 1982/10/25

Y1 - 1982/10/25

N2 - A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNA, binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatment, and thus appears to be due to the combined action of both heat and NEM-insensitive Co-eIF-2A, and heat and NEM-sensitive Co-eIF-2C activities. (3) [3H]GDP bound in eIF-2·[3H]GDP complex is rapidly displaced by unlabelled GTP during ternary complex formation., Co-eIF-2 stimulates. Met-tRNAf binding to eIF-2 even when added after the [3H]GDP from eIF-2·[3H]GDP has been completely displaced. This indicates that Co-eIF-2-stimulation is not due to GDP displacement from eIF-2·GDP. We propose that eIF-2 molecules become inactive in the presence of Mg2 and at high dilution, and Co-eIF-2 restores the inactive eIF-2 molecules into an active form.

AB - A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNA, binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatment, and thus appears to be due to the combined action of both heat and NEM-insensitive Co-eIF-2A, and heat and NEM-sensitive Co-eIF-2C activities. (3) [3H]GDP bound in eIF-2·[3H]GDP complex is rapidly displaced by unlabelled GTP during ternary complex formation., Co-eIF-2 stimulates. Met-tRNAf binding to eIF-2 even when added after the [3H]GDP from eIF-2·[3H]GDP has been completely displaced. This indicates that Co-eIF-2-stimulation is not due to GDP displacement from eIF-2·GDP. We propose that eIF-2 molecules become inactive in the presence of Mg2 and at high dilution, and Co-eIF-2 restores the inactive eIF-2 molecules into an active form.

UR - http://www.scopus.com/inward/record.url?scp=0020491287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020491287&partnerID=8YFLogxK

U2 - 10.1093/nar/10.20.6501

DO - 10.1093/nar/10.20.6501

M3 - Article

VL - 10

SP - 6501

EP - 6510

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 20

ER -