Abstract
The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNAf·EIF-1·GTP in the presence of Mg++ and is also required along with EIF-1 for AUG-directed Met-tRNAf binding to 40S ribosomes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-169 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 78 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 9 1977 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Cite this
Majumdar, A., Roy, R., Das, A., Dasgupta, A., & Gupta, N. K. (1977). Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNAf·EIF-1·GTP complex and Met-tRNAf binding to 40S ribosomes. Biochemical and Biophysical Research Communications, 78(1), 161-169. https://doi.org/10.1016/0006-291X(77)91235-9