Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNAf·EIF-1·GTP complex and Met-tRNAf binding to 40S ribosomes

A. Majumdar, R. Roy, A. Das, A. Dasgupta, N. K. Gupta

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNAf·EIF-1·GTP in the presence of Mg++ and is also required along with EIF-1 for AUG-directed Met-tRNAf binding to 40S ribosomes.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume78
Issue number1
DOIs
StatePublished - Sep 9 1977

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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