Protein translation and cell death

The role of rare tRNAs in biofilm formation and in activating dormant phage killer genes

Rodolfo García-Contreras, Xue Song Zhang, Younghoon Kim, Thomas Keith Wood

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We discovered previously that the small Escherichia coli proteins Hha (hemolysin expression modulating protein) and the adjacent, poorly-characterized YbaJ are important for biofilm formation; however, their roles been nebelous. Biofilms are intricate communities in which cell signaling often converts single cells into primitive tissues. Here we show that Hha decreases biofilm formation dramatically by repressing the transcription of rare codon tRNAs which serves to inhibit fimbriae production and by repressing to some extent transcription of fimbrial genes fimA and ihfA. In vivo binding studies show Hha binds to the rare condon tRNAs argU, ileX, ileY, and proL and to two prophage clusters D1P12 and CP4-57. Real-time PCR corroborated that Hha represses argU and proL, and Hha type I fimbriae repression is abolished by the addition of biofilm dipersal due to activation of prophage lytic genes rzpD, yfiZ, appY, and alpA and due to induction of CIpP/CIpX proteases which activate toxins by degrading antitoxins. YbaJ serves to mediate the toxicity of Hha. Hence, we have identified that a single prtein (Hha) can control biofilm formation by limiting fimbriae production as well by controlling cell death. The mechanism used by Hha is the control of translation via the availability of rare condo tRANs which reduces fimbriae production and activities prophage lytic genes. Therefore, Hha acts as a toxin in cojunction with co-transcribed YbaJ (TomB) that attenuates Hha toxicity.

Original languageEnglish (US)
Article numbere2394
JournalPLoS One
Volume3
Issue number6
DOIs
StatePublished - Jun 11 2008

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Bacteriophages
Biofilms
Protein Biosynthesis
Cell death
Transfer RNA
bacteriophages
biofilm
translation (genetics)
fimbriae
cell death
Prophages
Cell Death
Genes
Transcription
Proteins
genes
proteins
Toxicity
toxins
transcription (genetics)

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Protein translation and cell death: The role of rare tRNAs in biofilm formation and in activating dormant phage killer genes",
abstract = "We discovered previously that the small Escherichia coli proteins Hha (hemolysin expression modulating protein) and the adjacent, poorly-characterized YbaJ are important for biofilm formation; however, their roles been nebelous. Biofilms are intricate communities in which cell signaling often converts single cells into primitive tissues. Here we show that Hha decreases biofilm formation dramatically by repressing the transcription of rare codon tRNAs which serves to inhibit fimbriae production and by repressing to some extent transcription of fimbrial genes fimA and ihfA. In vivo binding studies show Hha binds to the rare condon tRNAs argU, ileX, ileY, and proL and to two prophage clusters D1P12 and CP4-57. Real-time PCR corroborated that Hha represses argU and proL, and Hha type I fimbriae repression is abolished by the addition of biofilm dipersal due to activation of prophage lytic genes rzpD, yfiZ, appY, and alpA and due to induction of CIpP/CIpX proteases which activate toxins by degrading antitoxins. YbaJ serves to mediate the toxicity of Hha. Hence, we have identified that a single prtein (Hha) can control biofilm formation by limiting fimbriae production as well by controlling cell death. The mechanism used by Hha is the control of translation via the availability of rare condo tRANs which reduces fimbriae production and activities prophage lytic genes. Therefore, Hha acts as a toxin in cojunction with co-transcribed YbaJ (TomB) that attenuates Hha toxicity.",
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Protein translation and cell death : The role of rare tRNAs in biofilm formation and in activating dormant phage killer genes. / García-Contreras, Rodolfo; Zhang, Xue Song; Kim, Younghoon; Wood, Thomas Keith.

In: PLoS One, Vol. 3, No. 6, e2394, 11.06.2008.

Research output: Contribution to journalArticle

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T2 - The role of rare tRNAs in biofilm formation and in activating dormant phage killer genes

AU - García-Contreras, Rodolfo

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AU - Kim, Younghoon

AU - Wood, Thomas Keith

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AB - We discovered previously that the small Escherichia coli proteins Hha (hemolysin expression modulating protein) and the adjacent, poorly-characterized YbaJ are important for biofilm formation; however, their roles been nebelous. Biofilms are intricate communities in which cell signaling often converts single cells into primitive tissues. Here we show that Hha decreases biofilm formation dramatically by repressing the transcription of rare codon tRNAs which serves to inhibit fimbriae production and by repressing to some extent transcription of fimbrial genes fimA and ihfA. In vivo binding studies show Hha binds to the rare condon tRNAs argU, ileX, ileY, and proL and to two prophage clusters D1P12 and CP4-57. Real-time PCR corroborated that Hha represses argU and proL, and Hha type I fimbriae repression is abolished by the addition of biofilm dipersal due to activation of prophage lytic genes rzpD, yfiZ, appY, and alpA and due to induction of CIpP/CIpX proteases which activate toxins by degrading antitoxins. YbaJ serves to mediate the toxicity of Hha. Hence, we have identified that a single prtein (Hha) can control biofilm formation by limiting fimbriae production as well by controlling cell death. The mechanism used by Hha is the control of translation via the availability of rare condo tRANs which reduces fimbriae production and activities prophage lytic genes. Therefore, Hha acts as a toxin in cojunction with co-transcribed YbaJ (TomB) that attenuates Hha toxicity.

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