Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior

Stineke van Houte, Vera I.D. Ros, Tom G. Mastenbroek, Nadia J. Vendrig, Kelli Hoover, Jeroen Spitzen, Monique M. van Oers

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Many parasites manipulate host behavior to increase the probability of transmission. To date, direct evidence for parasitic genes underlying such behavioral manipulations is scarce. Here we show that the baculovirus Autographa californica nuclear polyhedrovirus (AcMNPV) induces hyperactive behavior in Spodoptera exigua larvae at three days after infection. Furthermore, we identify the viral protein tyrosine phosphatase (ptp) gene as a key player in the induction of hyperactivity in larvae, and show that mutating the catalytic site of the encoded phosphatase enzyme prevents this induced behavior. Phylogenetic inference points at a lepidopteran origin of the ptp gene and shows that this gene is well-conserved in a group of related baculoviruses. Our study suggests that ptp-induced behavioral manipulation is an evolutionarily conserved strategy of this group of baculoviruses to enhance virus transmission, and represents an example of the extended phenotype concept. Overall, these data provide a firm base for a deeper understanding of the mechanisms behind baculovirus-induced insect behavior.

Original languageEnglish (US)
Article numbere46933
JournalPloS one
Volume7
Issue number10
DOIs
StatePublished - Oct 15 2012

Fingerprint

protein-tyrosine-phosphatase
Protein Tyrosine Phosphatases
Baculoviridae
Genes
Lepidoptera
Behavior Control
Larva
genes
Autographa californica
Autographa californica multiple nucleopolyhedrovirus
insect behavior
Spodoptera
Spodoptera exigua
larvae
viral proteins
virus transmission
Viral Proteins
Viruses
Phosphoric Monoester Hydrolases
active sites

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

van Houte, Stineke ; Ros, Vera I.D. ; Mastenbroek, Tom G. ; Vendrig, Nadia J. ; Hoover, Kelli ; Spitzen, Jeroen ; van Oers, Monique M. / Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior. In: PloS one. 2012 ; Vol. 7, No. 10.
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Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior. / van Houte, Stineke; Ros, Vera I.D.; Mastenbroek, Tom G.; Vendrig, Nadia J.; Hoover, Kelli; Spitzen, Jeroen; van Oers, Monique M.

In: PloS one, Vol. 7, No. 10, e46933, 15.10.2012.

Research output: Contribution to journalArticle

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