Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Genomic footprinting on the Drosophila hsp26 promoter in isolated nuclei has shown that a TATA box binding factor is present before and after induction by heat shock, while three of the seven heat shock consensus sequences 5' of the gene are occupied (presumably by heat shock factor, HSF) specifically on heat shock. The sites of HSF interaction are separated by greater than 200 bp of which approximately 150 bp are bound to the surface of a nucleosome. The juxtaposition of these various macromolecules on the DNA suggests a basis for the major DNase I hypersensitive site 5' of hsp26 and a novel tertiary structure for the promoter complex.

Original languageEnglish (US)
Pages (from-to)2191-2201
Number of pages11
JournalThe EMBO journal
Volume7
Issue number7
StatePublished - Jan 1 1988

Fingerprint

Deoxyribonuclease I
Drosophila
Shock
Hot Temperature
DNA
Proteins
TATA Box
Nucleosomes
Consensus Sequence
Macromolecules
Genes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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abstract = "Genomic footprinting on the Drosophila hsp26 promoter in isolated nuclei has shown that a TATA box binding factor is present before and after induction by heat shock, while three of the seven heat shock consensus sequences 5' of the gene are occupied (presumably by heat shock factor, HSF) specifically on heat shock. The sites of HSF interaction are separated by greater than 200 bp of which approximately 150 bp are bound to the surface of a nucleosome. The juxtaposition of these various macromolecules on the DNA suggests a basis for the major DNase I hypersensitive site 5' of hsp26 and a novel tertiary structure for the promoter complex.",
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Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter. / Thomas, Claire Madison; Elgin, S. C.

In: The EMBO journal, Vol. 7, No. 7, 01.01.1988, p. 2191-2201.

Research output: Contribution to journalArticle

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