Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.

G. H. Thomas, S. C. Elgin

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

Genomic footprinting on the Drosophila hsp26 promoter in isolated nuclei has shown that a TATA box binding factor is present before and after induction by heat shock, while three of the seven heat shock consensus sequences 5' of the gene are occupied (presumably by heat shock factor, HSF) specifically on heat shock. The sites of HSF interaction are separated by greater than 200 bp of which approximately 150 bp are bound to the surface of a nucleosome. The juxtaposition of these various macromolecules on the DNA suggests a basis for the major DNase I hypersensitive site 5' of hsp26 and a novel tertiary structure for the promoter complex.

Original languageEnglish (US)
Pages (from-to)2191-2201
Number of pages11
JournalThe EMBO journal
Volume7
Issue number7
DOIs
StatePublished - Jul 1988

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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