Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens

Sung Hyun Cho, Quoc Truong Hoang, Yoon Young Kim, Hyun Young Shin, Sung Han Ok, Jung Myung Bae, Jeong Sheop Shin

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Physcomitrella patens is a model plant for studying gene function using a knockout strategy. To establish a proteome database for P. patens, we resolved over 1,500 soluble proteins from gametophore and protonema tissues by two-dimensional electrophoresis (2-DE) and obtained peptide mass fingerprints (PMFs) by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Using expressed sequence tags (ESTs), we were able to predict the identities of 90 protein spots. Most of these were related to energy or primary metabolism. Comparative proteome analysis was used to identify proteins specific for each of the tissue types. One of these was a metallothionein type-2 (PpMT2) protein that was highly upregulated in gametophore tissue. PpMT2 was induced in both the gametophore and protonema following culture on solid media and in response to various abiotic stresses such as copper, cadmium, cold, indole-3-acetic acid, and ethylene. We suggest that PpMT2 is not only involved in metal binding and detoxification, but also in many biological aspects as a metal messenger or a protein with additional functions.

Original languageEnglish (US)
Pages (from-to)475-488
Number of pages14
JournalPlant Cell Reports
Volume25
Issue number5
DOIs
StatePublished - May 1 2006

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gametophores
Physcomitrella patens
metallothionein
proteome
abiotic stress
stress response
protonemata
proteins
metals
matrix-assisted laser desorption-ionization mass spectrometry
expressed sequence tags
indole acetic acid
electrophoresis
ethylene
cadmium
copper
peptides
metabolism
energy
tissues

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Cho, Sung Hyun ; Hoang, Quoc Truong ; Kim, Yoon Young ; Shin, Hyun Young ; Ok, Sung Han ; Bae, Jung Myung ; Shin, Jeong Sheop. / Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens. In: Plant Cell Reports. 2006 ; Vol. 25, No. 5. pp. 475-488.
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Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens. / Cho, Sung Hyun; Hoang, Quoc Truong; Kim, Yoon Young; Shin, Hyun Young; Ok, Sung Han; Bae, Jung Myung; Shin, Jeong Sheop.

In: Plant Cell Reports, Vol. 25, No. 5, 01.05.2006, p. 475-488.

Research output: Contribution to journalArticle

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