Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures

Corien Bakermans, Sandra L. Tollaksen, Carol S. Giometti, Curtis Wilkerson, James M. Tiedje, Michael F. Thomashow

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

It is crucial to examine the physiological processes of psychrophiles at temperatures below 4°C, particularly to facilitate extrapolation of laboratory results to in situ activity. Using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and -4°C of the eurypsychrophile Psychrobacter cryohalolentis K5 and report the first identification of cold inducible proteins (CIPs) present during growth at subzero temperatures. Growth temperature substantially reprogrammed the proteome; the relative abundance of 303 of the 618 protein spots detected (∼31% of the proteins at each growth temperature) varied significantly with temperature. Five CIPs were detected specifically at -4°C; their identities (AtpF, EF-Ts, TolC, Pcryo_1988, and FecA) suggested specific stress on energy production, protein synthesis, and transport during growth at subzero temperatures. The need for continual relief of low-temperature stress on these cellular processes was confirmed via identification of 22 additional CIPs whose abundance increased during growth at -4°C (relative to higher temperatures). Our data suggested that iron may be limiting during growth at subzero temperatures and that a cold-adapted allele was employed at -4°C for transport of iron. In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to -4°C.

Original languageEnglish (US)
Pages (from-to)343-354
Number of pages12
JournalExtremophiles
Volume11
Issue number2
DOIs
StatePublished - Mar 1 2007

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Psychrobacter
Proteomics
Temperature
Growth
Proteins
Iron
Physiological Phenomena
Protein Transport
Proteome
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine

Cite this

Bakermans, C., Tollaksen, S. L., Giometti, C. S., Wilkerson, C., Tiedje, J. M., & Thomashow, M. F. (2007). Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures. Extremophiles, 11(2), 343-354. https://doi.org/10.1007/s00792-006-0042-1
Bakermans, Corien ; Tollaksen, Sandra L. ; Giometti, Carol S. ; Wilkerson, Curtis ; Tiedje, James M. ; Thomashow, Michael F. / Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures. In: Extremophiles. 2007 ; Vol. 11, No. 2. pp. 343-354.
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Bakermans, C, Tollaksen, SL, Giometti, CS, Wilkerson, C, Tiedje, JM & Thomashow, MF 2007, 'Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures', Extremophiles, vol. 11, no. 2, pp. 343-354. https://doi.org/10.1007/s00792-006-0042-1

Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures. / Bakermans, Corien; Tollaksen, Sandra L.; Giometti, Carol S.; Wilkerson, Curtis; Tiedje, James M.; Thomashow, Michael F.

In: Extremophiles, Vol. 11, No. 2, 01.03.2007, p. 343-354.

Research output: Contribution to journalArticle

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AU - Tollaksen, Sandra L.

AU - Giometti, Carol S.

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AU - Tiedje, James M.

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AB - It is crucial to examine the physiological processes of psychrophiles at temperatures below 4°C, particularly to facilitate extrapolation of laboratory results to in situ activity. Using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and -4°C of the eurypsychrophile Psychrobacter cryohalolentis K5 and report the first identification of cold inducible proteins (CIPs) present during growth at subzero temperatures. Growth temperature substantially reprogrammed the proteome; the relative abundance of 303 of the 618 protein spots detected (∼31% of the proteins at each growth temperature) varied significantly with temperature. Five CIPs were detected specifically at -4°C; their identities (AtpF, EF-Ts, TolC, Pcryo_1988, and FecA) suggested specific stress on energy production, protein synthesis, and transport during growth at subzero temperatures. The need for continual relief of low-temperature stress on these cellular processes was confirmed via identification of 22 additional CIPs whose abundance increased during growth at -4°C (relative to higher temperatures). Our data suggested that iron may be limiting during growth at subzero temperatures and that a cold-adapted allele was employed at -4°C for transport of iron. In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to -4°C.

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