Proteomic and phosphoproteomic analyses reveal extensive phosphorylation of regulatory proteins in developing rice anthers

Juanying Ye, Zaibao Zhang, Haifei Long, Zhimin Zhang, Yue Hong, Xumin Zhang, Chenjiang You, Wanqi Liang, Hong Ma, Pingli Lu

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Anther development, particularly around the time of meiosis, is extremely crucial for plant sexual reproduction. Meanwhile, cell-to-cell communication between somatic (especial tapetum) cells and meiocytes are important for both somatic anther development and meiosis. To investigate possible molecular mechanisms modulating protein activities during anther development, we applied high-resolution mass spectrometry-based proteomic and phosphoproteomic analyses for developing rice (Oryza sativa) anthers around the time of meiosis (RAM). In total, we identified 4984 proteins and 3203 phosphoproteins with 8973 unique phosphorylation sites (p-sites). Among those detected here, 1544 phosphoproteins are currently absent in the Plant Protein Phosphorylation DataBase (P3DB), substantially enriching plant phosphorylation information. Mapman enrichment analysis showed that 'DNA repair','transcription regulation' and 'signaling' related proteins were overrepresented in the phosphorylated proteins. Ten genetically identified rice meiotic proteins were detected to be phosphorylated at a total of 25 p-sites; moreover more than 400 meiotically expressed proteins were revealed to be phosphorylated and their phosphorylation sites were precisely assigned. 163 putative secretory proteins, possibly functioning in cell-to-cell communication, are also phosphorylated. Furthermore, we showed that DNA synthesis, RNA splicing and RNA-directed DNA methylation pathways are extensively affected by phosphorylation. In addition, our data support 46 kinase-substrate pairs predicted by the rice Kinase-Protein Interaction Map, with SnRK1 substrates highly enriched. Taken together, our data revealed extensive protein phosphorylation during anther development, suggesting an important post-translational modification affecting protein activity. Significance Statement Meiosis and subsequent pollen development is crucial for plant reproduction. Here we used high-resolution proteomics and phosphoproteomics to provide a community resource on the abundance and phosphorylation status of thousands of rice proteins in developing anthers, which will help in elucidating the molecular mechanisms underlying anther development and meiosis.

Original languageEnglish (US)
Pages (from-to)527-544
Number of pages18
JournalPlant Journal
Volume84
Issue number3
DOIs
StatePublished - Nov 2015

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Proteomic and phosphoproteomic analyses reveal extensive phosphorylation of regulatory proteins in developing rice anthers'. Together they form a unique fingerprint.

Cite this