Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

Arunangshu Das, James D. Bortner, Cesar Aliaga, Timothy Cooper, Anne Stanley, Bruce Stanley, Chandra Belani, Karam El-Bayoumy

Research output: Contribution to journalArticle

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Abstract

We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.

Original languageEnglish (US)
Pages (from-to)23-33
Number of pages11
JournalEuPA Open Proteomics
Volume9
DOIs
StatePublished - Dec 1 2015

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Biomarkers
Adenoma
Proteomics
Hyperplasia
Lung
Proteins
Alcohol Oxidoreductases
Tobacco
Annexin A5
Early Detection of Cancer
Carcinogens
Lung Neoplasms
Carcinogenesis
Adenocarcinoma

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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title = "Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection",
abstract = "We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95{\%} confidence and a Local False Discovery Rate estimation less than 5{\%}. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.",
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AU - Das, Arunangshu

AU - Bortner, James D.

AU - Aliaga, Cesar

AU - Cooper, Timothy

AU - Stanley, Anne

AU - Stanley, Bruce

AU - Belani, Chandra

AU - El-Bayoumy, Karam

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