Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

Arunangshu Das, James D. Bortner, Cesar Aliaga, Timothy Cooper, Anne Stanley, Bruce A. Stanley, Chandra P. Belani, Karam El-Bayoumy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.

Original languageEnglish (US)
Pages (from-to)23-33
Number of pages11
JournalEuPA Open Proteomics
Volume9
DOIs
StatePublished - Dec 1 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry

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