Abstract
Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg −1 protein to 6 µmol mg −1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.
| Original language | English (US) |
|---|---|
| Article number | 435 |
| Journal | 3 Biotech |
| Volume | 8 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2018 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Environmental Science (miscellaneous)
- Agricultural and Biological Sciences (miscellaneous)
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Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113. / Zakaria, Muhammad Azman; Mohd Yusoff, Mohd Zulkhairi; Zakaria, Mohd Rafein; Hassan, Mohd Ali; Wood, Thomas Keith; Maeda, Toshinari.
In: 3 Biotech, Vol. 8, No. 10, 435, 01.10.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113
AU - Zakaria, Muhammad Azman
AU - Mohd Yusoff, Mohd Zulkhairi
AU - Zakaria, Mohd Rafein
AU - Hassan, Mohd Ali
AU - Wood, Thomas Keith
AU - Maeda, Toshinari
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg −1 protein to 6 µmol mg −1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.
AB - Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg −1 protein to 6 µmol mg −1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.
UR - http://www.scopus.com/inward/record.url?scp=85054169625&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054169625&partnerID=8YFLogxK
U2 - 10.1007/s13205-018-1461-2
DO - 10.1007/s13205-018-1461-2
M3 - Article
C2 - 30306004
AN - SCOPUS:85054169625
VL - 8
JO - 3 Biotech
JF - 3 Biotech
SN - 2190-572X
IS - 10
M1 - 435
ER -