TY - JOUR
T1 - Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113
AU - Zakaria, Muhammad Azman
AU - Mohd Yusoff, Mohd Zulkhairi
AU - Zakaria, Mohd Rafein
AU - Hassan, Mohd Ali
AU - Wood, Thomas K.
AU - Maeda, Toshinari
N1 - Funding Information:
We express our gratitude to the National Institute of Genetics (Japan) for providing the Keio mutant and ASKA strains. The authors would like to thank the Ministry of Higher Education, Malaysia for providing Fundamental Research Grant Scheme (02-01-14-1401FR), the School of Graduate Studies, Universiti Putra Malaysia and Japan Student Services Organisation for the scholarship to Muhammad Azman Zakaria during this study. The authors declare that there have no conflict of interest in relation to this article.
Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg−1 protein to 6 µmol mg−1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.
AB - Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg−1 protein to 6 µmol mg−1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.
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U2 - 10.1007/s13205-018-1461-2
DO - 10.1007/s13205-018-1461-2
M3 - Article
C2 - 30306004
AN - SCOPUS:85054169625
VL - 8
JO - 3 Biotech
JF - 3 Biotech
SN - 2190-572X
IS - 10
M1 - 435
ER -