Pseudomonas sp. LSW25R, antagonistic to plant pathogens, promoted plant growth, and reduced blossom-end rot of tomato fruits in a hydroponic system

Sang Woo Lee, Il Pyung Ahn, Sang Yeon Sim, Sue Yeon Lee, Myeon Whoon Seo, Soonok Kim, Sook Young Park, Yong Hwan Lee, Seogchan Kang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants at 108 cfu·ml-1, and increased the plant growth of eggplants and peppers at 104 cfu·ml-1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover, densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9. 3 × 103 cfu·g-1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system, LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalEuropean Journal of Plant Pathology
Volume126
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

hydroponics
plant pathogens
Pseudomonas
plant growth
tomatoes
fruits
growth promotion
eggplants
pepper
Pseudomonas corrugata
uptake mechanisms
calcium
rifampicin
seedlings
pathogens
nutrients
plant veins
Botrytis cinerea
scanning electron microscopes
internal transcribed spacers

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

Cite this

Lee, Sang Woo ; Ahn, Il Pyung ; Sim, Sang Yeon ; Lee, Sue Yeon ; Seo, Myeon Whoon ; Kim, Soonok ; Park, Sook Young ; Lee, Yong Hwan ; Kang, Seogchan. / Pseudomonas sp. LSW25R, antagonistic to plant pathogens, promoted plant growth, and reduced blossom-end rot of tomato fruits in a hydroponic system. In: European Journal of Plant Pathology. 2010 ; Vol. 126, No. 1. pp. 1-11.
@article{18d6dd13d22f458b8b0bf3ff4a569350,
title = "Pseudomonas sp. LSW25R, antagonistic to plant pathogens, promoted plant growth, and reduced blossom-end rot of tomato fruits in a hydroponic system",
abstract = "A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants at 108 cfu·ml-1, and increased the plant growth of eggplants and peppers at 104 cfu·ml-1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover, densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9. 3 × 103 cfu·g-1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system, LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake.",
author = "Lee, {Sang Woo} and Ahn, {Il Pyung} and Sim, {Sang Yeon} and Lee, {Sue Yeon} and Seo, {Myeon Whoon} and Soonok Kim and Park, {Sook Young} and Lee, {Yong Hwan} and Seogchan Kang",
year = "2010",
month = "1",
day = "1",
doi = "10.1007/s10658-009-9514-3",
language = "English (US)",
volume = "126",
pages = "1--11",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
publisher = "Springer Netherlands",
number = "1",

}

Pseudomonas sp. LSW25R, antagonistic to plant pathogens, promoted plant growth, and reduced blossom-end rot of tomato fruits in a hydroponic system. / Lee, Sang Woo; Ahn, Il Pyung; Sim, Sang Yeon; Lee, Sue Yeon; Seo, Myeon Whoon; Kim, Soonok; Park, Sook Young; Lee, Yong Hwan; Kang, Seogchan.

In: European Journal of Plant Pathology, Vol. 126, No. 1, 01.01.2010, p. 1-11.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pseudomonas sp. LSW25R, antagonistic to plant pathogens, promoted plant growth, and reduced blossom-end rot of tomato fruits in a hydroponic system

AU - Lee, Sang Woo

AU - Ahn, Il Pyung

AU - Sim, Sang Yeon

AU - Lee, Sue Yeon

AU - Seo, Myeon Whoon

AU - Kim, Soonok

AU - Park, Sook Young

AU - Lee, Yong Hwan

AU - Kang, Seogchan

PY - 2010/1/1

Y1 - 2010/1/1

N2 - A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants at 108 cfu·ml-1, and increased the plant growth of eggplants and peppers at 104 cfu·ml-1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover, densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9. 3 × 103 cfu·g-1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system, LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake.

AB - A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants at 108 cfu·ml-1, and increased the plant growth of eggplants and peppers at 104 cfu·ml-1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover, densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9. 3 × 103 cfu·g-1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system, LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake.

UR - http://www.scopus.com/inward/record.url?scp=67650675878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650675878&partnerID=8YFLogxK

U2 - 10.1007/s10658-009-9514-3

DO - 10.1007/s10658-009-9514-3

M3 - Article

AN - SCOPUS:67650675878

VL - 126

SP - 1

EP - 11

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 1

ER -