Three Gram-negative, non-motile, non-pigmented, oxidase-positive coccobacilli capable of growth at temperatures from -10 to 30 °C and salinities of 0 to 1.7 M NaCl were isolated from Siberian permafrost and characterized. Both 16S rRNA and gyrB gene sequencing studies placed the isolates in the Gammaproteobacteria within the genus Psychrobacter. However, with higher bootstrap values and reproducible tree topologies, gyrB represented a more reliable phylogenetic marker for the taxonomy of Psychrobacter species. DNA-DNA hybridization data supported gyrB tree topologies and established two relatedness groups within the three isolates; neither of these groups was related at the species level to any previously described Psychrobacter species. The two groups of isolates could be differentiated phenotypically from 13 previously described Psychrobacter species using API strips. These results support the existence of two novel species of Psychrobacter, for which we propose the names Psychrobacter cryohalolentis sp. nov. (type strain K5T = DSM 17306T = VKM B-2378T) and Psychrobacter arcticus sp. nov. (type strain 273-4T = DSM 17307T = VKM B-2377T).
|Original language||English (US)|
|Number of pages||7|
|Journal||International journal of systematic and evolutionary microbiology|
|State||Published - Jun 2006|
All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics