Puf3 participates in ribosomal biogenesis in malaria parasites

Xiaoying Liang, Kevin J. Hart, Gang Dong, Faiza A. Siddiqui, Aswathy Sebastian, Xiaolian Li, Istvan Albert, Jun Miao, Scott E. Lindner, Liwang Cui

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, we characterized the Puf family gene member Puf3 in the malaria parasites Plasmodium falciparum and Plasmodium yoelii. Secondary structure prediction suggested that the RNA-binding domains of the Puf3 proteins consisted of 11 pumilio repeats that were similar to those in the human Puf-A (also known as PUM3) and Saccharomyces cerevisiae Puf6 proteins, which are involved in ribosome biogenesis. Neither P. falciparum (Pf)Puf3 nor P. yoelii (Py)Puf3 could be genetically disrupted, suggesting they may be essential for the intraerythrocytic developmental cycle. Cellular fractionation of PfPuf3 in the asexual stages revealed preferential partitioning to the nuclear fraction, consistentwith nuclear localization of PfPuf3::GFP and PyPuf3::GFP as detected by immunofluorescence. Furthermore, PfPuf3 colocalized with the nucleolar marker PfNop1, demonstrating that PfPuf3 is a nucleolar protein in the asexual stages. We found, however, that PyPuf3 changed its localization from being nucleolar to being present in cytosolic puncta in the mosquito and liver stages, which may reflect alternative functions in these stages. Affinity purification of molecules that associated with a PTP-tagged variant of PfPuf3 revealed 31 proteins associated with the 60S ribosome, and an enrichment of 28S rRNA and internal transcribed spacer 2 sequences. Taken together, these results suggest an essential function forPfPuf3 in ribosomal biogenesis.

Original languageEnglish (US)
Article numberjcs212597
JournalJournal of Cell Science
Volume131
Issue number6
DOIs
StatePublished - Mar 1 2018

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Plasmodium yoelii
Ribosomes
Malaria
Parasites
Saccharomyces cerevisiae Proteins
Falciparum Malaria
Plasmodium falciparum
Nuclear Proteins
Culicidae
Fluorescent Antibody Technique
Liver
Genes
Proteins
RNA Recognition Motif Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Liang, X., Hart, K. J., Dong, G., Siddiqui, F. A., Sebastian, A., Li, X., ... Cui, L. (2018). Puf3 participates in ribosomal biogenesis in malaria parasites. Journal of Cell Science, 131(6), [jcs212597]. https://doi.org/10.1242/jcs.212597
Liang, Xiaoying ; Hart, Kevin J. ; Dong, Gang ; Siddiqui, Faiza A. ; Sebastian, Aswathy ; Li, Xiaolian ; Albert, Istvan ; Miao, Jun ; Lindner, Scott Eugene ; Cui, Liwang. / Puf3 participates in ribosomal biogenesis in malaria parasites. In: Journal of Cell Science. 2018 ; Vol. 131, No. 6.
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Liang, X, Hart, KJ, Dong, G, Siddiqui, FA, Sebastian, A, Li, X, Albert, I, Miao, J, Lindner, SE & Cui, L 2018, 'Puf3 participates in ribosomal biogenesis in malaria parasites', Journal of Cell Science, vol. 131, no. 6, jcs212597. https://doi.org/10.1242/jcs.212597

Puf3 participates in ribosomal biogenesis in malaria parasites. / Liang, Xiaoying; Hart, Kevin J.; Dong, Gang; Siddiqui, Faiza A.; Sebastian, Aswathy; Li, Xiaolian; Albert, Istvan; Miao, Jun; Lindner, Scott E.; Cui, Liwang.

In: Journal of Cell Science, Vol. 131, No. 6, jcs212597, 01.03.2018.

Research output: Contribution to journalArticle

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Liang X, Hart KJ, Dong G, Siddiqui FA, Sebastian A, Li X et al. Puf3 participates in ribosomal biogenesis in malaria parasites. Journal of Cell Science. 2018 Mar 1;131(6). jcs212597. https://doi.org/10.1242/jcs.212597