Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes

Leah A. Mitchell, Jun Gao, Randy Lee Vander Wal, Andrew Gigliotti, Scott W. Burchiel, Jacob D. McDonald

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

Inhalation of multiwalled carbon nanotubes (MWCNTs) at particle concentrations ranging from 0.3 to 5 mg/m3 did not result in significant lung inflammation or tissue damage, but caused systemic immune function alterations. C57BL/6 adult (10- to 12-week) male mice were exposed by whole-body inhalation to control air or 0.3, 1, or 5 mg/m3 respirable aggregates of MWCNTs for 7 or 14 days (6 h/day). Histopathology of lungs from exposed animals showed alveolar macrophages containing black particles; however, there was no inflammation or tissue damage observed. Bronchial alveolar lavage fluid also demonstrated particle-laden macrophages; however, white blood cell counts were not increased compared to controls. MWCNT exposures to 0.3 mg/3 and higher particle concentrations caused nonmonotonic systemic immunosuppression after 14 days but not after 7 days. Immunosuppression was characterized by reduced T-cell-dependent antibody response to sheep erythrocytes as well as T-cell proliferative ability in presence of mitogen, Concanavalin A. Assessment of nonspecific natural killer (NK) cell activity showed that animals exposed to 1 mg/m3 had decreased NK cell function. Gene expression analysis of selected cytokines and an indicator of oxidative stress were assessed in lung tissue and spleen. No changes in gene expression were observed in lung; however, interleukin-10 (IL-10) and NAD(P)H oxidoreductase 1 mRNA levels were increased in spleen.

Original languageEnglish (US)
Pages (from-to)203-214
Number of pages12
JournalToxicological Sciences
Volume100
Issue number1
DOIs
StatePublished - Nov 1 2007

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Carbon Nanotubes
Multiwalled carbon nanotubes (MWCN)
T-cells
Tissue
Gene expression
Natural Killer Cells
Lung
Immunosuppression
Inhalation
Animals
Spleen
T-Lymphocytes
Gene Expression
Oxidative stress
Macrophages
Bronchoalveolar Lavage Fluid
Alveolar Macrophages
Concanavalin A
Leukocyte Count
Mitogens

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Mitchell, L. A., Gao, J., Vander Wal, R. L., Gigliotti, A., Burchiel, S. W., & McDonald, J. D. (2007). Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. Toxicological Sciences, 100(1), 203-214. https://doi.org/10.1093/toxsci/kfm196
Mitchell, Leah A. ; Gao, Jun ; Vander Wal, Randy Lee ; Gigliotti, Andrew ; Burchiel, Scott W. ; McDonald, Jacob D. / Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. In: Toxicological Sciences. 2007 ; Vol. 100, No. 1. pp. 203-214.
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Mitchell, LA, Gao, J, Vander Wal, RL, Gigliotti, A, Burchiel, SW & McDonald, JD 2007, 'Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes', Toxicological Sciences, vol. 100, no. 1, pp. 203-214. https://doi.org/10.1093/toxsci/kfm196

Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. / Mitchell, Leah A.; Gao, Jun; Vander Wal, Randy Lee; Gigliotti, Andrew; Burchiel, Scott W.; McDonald, Jacob D.

In: Toxicological Sciences, Vol. 100, No. 1, 01.11.2007, p. 203-214.

Research output: Contribution to journalArticle

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