Purification and characterization of an extracellular lipase from a newly isolated thermophilic Bacillus pumilus

Joyce Jose, G. Muraleedhara Kurup

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13 Scopus citations

Abstract

An extracellular lipase was isolated and purified from a bacterium and is the first report on the isolation and purification of lipase from the species B. pumilus. Effect of temperature, pH and composition of culture medium were optimized for maximum lipase production. The enzyme was purified and the purity was found to be 98%. The Km value of the enzyme was 1.75 x 10-2 mg. and it was found to be a monomer by SDS PAGE. This lipase was found to be alkaline and thermostable and was not a metalloprotein as evidenced from EDTA treatment. Immobilized whole cells were found to be more stable than the pure enzyme.

Original languageEnglish (US)
Pages (from-to)1213-1217
Number of pages5
JournalIndian Journal of Experimental Biology
Volume37
Issue number12
StatePublished - Dec 1 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Cell Biology

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