Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676

Jianming Jin, Seung Ryel Baek, Kyung Rim Lee, Jungkwan Lee, Sung Hwan Yun, Seogchan Kang, Yin Won Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at 25°C, the culture was extracted with chloroform. Apicidins were purified through a reverse phase C18 silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin D2 from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin D2 showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables

Original languageEnglish (US)
Pages (from-to)417-422
Number of pages6
JournalPlant Pathology Journal
Volume24
Issue number4
DOIs
StatePublished - Dec 1 2008

Fingerprint

Fusarium incarnatum
phytotoxicity
Fusarium
Fusarium equiseti
histone deacetylase
wheat
silica gel
chloroform
translation (genetics)
mechanism of action
weeds
vegetables
biosynthesis
seedlings
methodology
genes
testing

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Jin, Jianming ; Baek, Seung Ryel ; Lee, Kyung Rim ; Lee, Jungkwan ; Yun, Sung Hwan ; Kang, Seogchan ; Lee, Yin Won. / Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676. In: Plant Pathology Journal. 2008 ; Vol. 24, No. 4. pp. 417-422.
@article{b6efc15ee78949aabbfd376f34cbac24,
title = "Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676",
abstract = "Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at 25°C, the culture was extracted with chloroform. Apicidins were purified through a reverse phase C18 silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin D2 from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin D2 showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables",
author = "Jianming Jin and Baek, {Seung Ryel} and Lee, {Kyung Rim} and Jungkwan Lee and Yun, {Sung Hwan} and Seogchan Kang and Lee, {Yin Won}",
year = "2008",
month = "12",
day = "1",
doi = "10.5423/PPJ.2008.24.4.417",
language = "English (US)",
volume = "24",
pages = "417--422",
journal = "Plant Pathology Journal",
issn = "1598-2254",
publisher = "Korean Society of Plant Pathology",
number = "4",

}

Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676. / Jin, Jianming; Baek, Seung Ryel; Lee, Kyung Rim; Lee, Jungkwan; Yun, Sung Hwan; Kang, Seogchan; Lee, Yin Won.

In: Plant Pathology Journal, Vol. 24, No. 4, 01.12.2008, p. 417-422.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676

AU - Jin, Jianming

AU - Baek, Seung Ryel

AU - Lee, Kyung Rim

AU - Lee, Jungkwan

AU - Yun, Sung Hwan

AU - Kang, Seogchan

AU - Lee, Yin Won

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at 25°C, the culture was extracted with chloroform. Apicidins were purified through a reverse phase C18 silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin D2 from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin D2 showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables

AB - Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at 25°C, the culture was extracted with chloroform. Apicidins were purified through a reverse phase C18 silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin D2 from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin D2 showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables

UR - http://www.scopus.com/inward/record.url?scp=77951165713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951165713&partnerID=8YFLogxK

U2 - 10.5423/PPJ.2008.24.4.417

DO - 10.5423/PPJ.2008.24.4.417

M3 - Article

AN - SCOPUS:77951165713

VL - 24

SP - 417

EP - 422

JO - Plant Pathology Journal

JF - Plant Pathology Journal

SN - 1598-2254

IS - 4

ER -