Purification of a cell-surface receptor for surfactant protein A

Zissis Chroneos, Rasul Abdolrasulnia, Jeffrey A. Whitsett, W. R. Rice, Virginia L. Shepherd

Research output: Contribution to journalArticle

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Abstract

In the present report we have characterized the binding of surfactant protein A (SP-A) to bone marrow-derived macrophages, U937 cells, alveolar macrophages, and type II epithelial cells. The binding of SP-A to all cell types is Ca 2+-dependent and trypsin-sensitive, but type II cells express distinct Ca 2+-independent binding sites. The binding of SP-A to macrophages is independent of known cell surface carbohydrate-specific receptors and of glycoconjugate binding sites on the surface of the cells and is distinct from binding to C1q receptors. Based on ligand blot analysis, both type II cells and macrophages express a 210-kDa SP-A-binding protein. The 210-kDa protein was purified to apparent homogeneity from U937 macrophage membranes using affinity chromatography with noncovalently immobilized surfactant protein A, and was purified from rat lung by differential detergent and salt extraction of isolated rat lung membranes. Polyclonal antibodies against the rat lung SP-A-binding protein inhibit binding of SP-A to both type II cells and macrophages, indicating that the 210-kDa protein is expressed on the cell surface. The polyclonal antibodies also block the SP-A-mediated inhibition of phospholipid secretion by type II cells, indicating that the 210-kDa protein is a functional cell-surface receptor on type II cells. In a separate report we have determined that antibodies to the SP-A receptor block the SP-A- mediated uptake of Mycobacterium bovis, indicating that the macrophage SP-A receptor is involved in SP-A-mediated clearance of pathogens.

Original languageEnglish (US)
Pages (from-to)16375-16383
Number of pages9
JournalJournal of Biological Chemistry
Volume271
Issue number27
DOIs
StatePublished - Jul 29 1996

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Pulmonary Surfactant-Associated Protein A
Cell Surface Receptors
Purification
Macrophages
Carrier Proteins
Protein Binding
Rats
Antibodies
Pulmonary Surfactant-Associated Proteins
Binding Sites
Immobilized Proteins
Membranes
Affinity chromatography
Lung
U937 Cells
Proteins
Glycoconjugates
Alveolar Macrophages
Pathogens
Mycobacterium bovis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chroneos, Z., Abdolrasulnia, R., Whitsett, J. A., Rice, W. R., & Shepherd, V. L. (1996). Purification of a cell-surface receptor for surfactant protein A. Journal of Biological Chemistry, 271(27), 16375-16383. https://doi.org/10.1074/jbc.271.27.16375
Chroneos, Zissis ; Abdolrasulnia, Rasul ; Whitsett, Jeffrey A. ; Rice, W. R. ; Shepherd, Virginia L. / Purification of a cell-surface receptor for surfactant protein A. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 27. pp. 16375-16383.
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Chroneos, Z, Abdolrasulnia, R, Whitsett, JA, Rice, WR & Shepherd, VL 1996, 'Purification of a cell-surface receptor for surfactant protein A', Journal of Biological Chemistry, vol. 271, no. 27, pp. 16375-16383. https://doi.org/10.1074/jbc.271.27.16375

Purification of a cell-surface receptor for surfactant protein A. / Chroneos, Zissis; Abdolrasulnia, Rasul; Whitsett, Jeffrey A.; Rice, W. R.; Shepherd, Virginia L.

In: Journal of Biological Chemistry, Vol. 271, No. 27, 29.07.1996, p. 16375-16383.

Research output: Contribution to journalArticle

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T1 - Purification of a cell-surface receptor for surfactant protein A

AU - Chroneos, Zissis

AU - Abdolrasulnia, Rasul

AU - Whitsett, Jeffrey A.

AU - Rice, W. R.

AU - Shepherd, Virginia L.

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