Purification of dendritic cell and macrophage subsets from the normal mouse small intestine

Balázs Koscsó, Kavitha Gowda, Todd D. Schell, Milena Bogunovic

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Mononuclear phagocytes are essential for protecting against pathogens breaching the intestinal mucosa and maintaining the integrity of the gastrointestinal tract. The mononuclear phagocyte family of the healthy intestine is represented by a small population of hematopoietic cells including dendritic cells and macrophages. Distinct mononuclear phagocyte subsets strategically accumulate within and below the mucosal epithelium and are distributed in the submucosa and muscularis externa. Shaped by its unique microenvironment, each mononuclear phagocyte subset is developmentally and functionally unique and phenotypically distinct. Here we summarize our recent advances on identifying and purifying various intestinal mononuclear phagocyte subsets by flow cytometry in the context of their developmental properties and location within the intestinal tissue.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalJournal of Immunological Methods
Volume421
DOIs
StatePublished - Jun 1 2015

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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