The amount of S adenosyl L methionine decarboxylase present in rat liver was enhanced 17 fold by administration of methylglyoxal bis(guanylhydrazone), a specific inhibitor of the enzyme. The enzyme was purified 1400 fold in 50% yield from such liver extracts by chromatography on columns of the inhibitor bound to Sepharose. The purified enzyme had no spermidine synthetase activity.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology