Purification of the serum acid-stable insulin-like growth factor binding protein from the pig (Sus scrofa)

Paul E. Walton, Robert C. Baxter, B. Dan Burleigh, Terry D. Etherton

Research output: Contribution to journalArticle

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1. 1. An acid-stable IGF binding protein was isolated and purified from porcine serum. 2. 2. The protein comprised two major species with Mrs of 45 and 41 kDa determined using SDS-PAGE under reducing conditions. 3. 3. The IGFBP preparation specifically bound both IGF-I and II. 4. 4. Four distinct protein bands (Mrs of 23, 45, 50 and 75 kDa) in the porcine IGFBP preparation specifically bound radiolabelled IGF-I. 5. 5. The porcine IGFBP exhibited sequence homology with IGFBPs from human plasma and rat serum. 6. 6. This is the first report of the purification and characterization of the acid-stable IGFBP from porcine serum.

Original languageEnglish (US)
Pages (from-to)561-567
Number of pages7
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Issue number3
Publication statusPublished - Jan 1 1989


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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