1. 1. An acid-stable IGF binding protein was isolated and purified from porcine serum. 2. 2. The protein comprised two major species with Mrs of 45 and 41 kDa determined using SDS-PAGE under reducing conditions. 3. 3. The IGFBP preparation specifically bound both IGF-I and II. 4. 4. Four distinct protein bands (Mrs of 23, 45, 50 and 75 kDa) in the porcine IGFBP preparation specifically bound radiolabelled IGF-I. 5. 5. The porcine IGFBP exhibited sequence homology with IGFBPs from human plasma and rat serum. 6. 6. This is the first report of the purification and characterization of the acid-stable IGFBP from porcine serum.
|Original language||English (US)|
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - Jan 1 1989|
All Science Journal Classification (ASJC) codes
- Molecular Biology