Pygidial glands of Harpalus pensylvanicus (Coleoptera: Carabidae) contain resilin-rich structures

Adam M. Rork, Istvan Miko, Tanya Renner

Research output: Contribution to journalArticle

Abstract

The pygidial gland system is a key innovation in adephagan beetles, producing, storing, and spraying defensive chemical compounds. As the source of defensive chemical production and storage, the pygidial gland system experiences severe chemical stress which challenges the integrity of the entire gland system. Here, we utilize autofluorescence-based confocal laser scanning microscopy to examine the morphology of pygidial gland secretory lobes and collecting ductules in a common Pennsylvanian harpaline species, Harpalus pensylvanicus. The glandular units are composed of type-III exocrine cells which empty into resilin-rich ductules, which themselves lead into a larger resilin-rich collecting duct, and ultimately the pygidial reservoir pump. We also utilize histological staining with toluidine blue and brightfield imaging to provide additional support for the presence of resilin in the collecting duct, as toluidine blue has been shown to stain resilin without metachromasia. We hypothesize that the high resilin content of the collecting ducts might be a widespread key evolutionary adaptation to prevent damage caused by physical and chemical stress generated in pump-containing insect exocrine gland systems.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalArthropod Structure and Development
Volume49
DOIs
StatePublished - Mar 1 2019

Fingerprint

Harpalus
toluidine blue
Beetles
Carabidae
pumps
exocrine glands
Coleoptera
evolutionary adaptation
chemical compounds
pump
confocal laser scanning microscopy
Tolonium Chloride
spraying
dyes
chemical compound
image analysis
Pennsylvanian
insects
microscopy
Exocrine Glands

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Developmental Biology
  • Insect Science

Cite this

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title = "Pygidial glands of Harpalus pensylvanicus (Coleoptera: Carabidae) contain resilin-rich structures",
abstract = "The pygidial gland system is a key innovation in adephagan beetles, producing, storing, and spraying defensive chemical compounds. As the source of defensive chemical production and storage, the pygidial gland system experiences severe chemical stress which challenges the integrity of the entire gland system. Here, we utilize autofluorescence-based confocal laser scanning microscopy to examine the morphology of pygidial gland secretory lobes and collecting ductules in a common Pennsylvanian harpaline species, Harpalus pensylvanicus. The glandular units are composed of type-III exocrine cells which empty into resilin-rich ductules, which themselves lead into a larger resilin-rich collecting duct, and ultimately the pygidial reservoir pump. We also utilize histological staining with toluidine blue and brightfield imaging to provide additional support for the presence of resilin in the collecting duct, as toluidine blue has been shown to stain resilin without metachromasia. We hypothesize that the high resilin content of the collecting ducts might be a widespread key evolutionary adaptation to prevent damage caused by physical and chemical stress generated in pump-containing insect exocrine gland systems.",
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Pygidial glands of Harpalus pensylvanicus (Coleoptera : Carabidae) contain resilin-rich structures. / Rork, Adam M.; Miko, Istvan; Renner, Tanya.

In: Arthropod Structure and Development, Vol. 49, 01.03.2019, p. 19-25.

Research output: Contribution to journalArticle

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