Pyocyanin restricts social cheating in Pseudomonas aeruginosa

Paulina Castañeda-Tamez, Jimena Ramírez-Peris, Judith Pérez-Velázquez, Christina Kuttler, Ammar Jalalimanesh, Miguel Saucedo-Mora, J. Guillermo Jiménez-Cortés, Toshinari Maeda, Yael González, María Tomás, Thomas K. Wood, Rodolfo García-Contreras

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.

Original languageEnglish (US)
Article number1348
JournalFrontiers in Microbiology
Volume9
Issue numberJUN
DOIs
StatePublished - Jun 27 2018

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Pyocyanine
Quorum Sensing
Pseudomonas aeruginosa
Bacteria
Exopeptidases
Siderophores
Acinetobacter baumannii
Poisons
Virulence Factors
Population
Oxidation-Reduction
Reactive Oxygen Species
Oxidative Stress
Theoretical Models

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Castañeda-Tamez, P., Ramírez-Peris, J., Pérez-Velázquez, J., Kuttler, C., Jalalimanesh, A., Saucedo-Mora, M., ... García-Contreras, R. (2018). Pyocyanin restricts social cheating in Pseudomonas aeruginosa. Frontiers in Microbiology, 9(JUN), [1348]. https://doi.org/10.3389/fmicb.2018.01348
Castañeda-Tamez, Paulina ; Ramírez-Peris, Jimena ; Pérez-Velázquez, Judith ; Kuttler, Christina ; Jalalimanesh, Ammar ; Saucedo-Mora, Miguel ; Jiménez-Cortés, J. Guillermo ; Maeda, Toshinari ; González, Yael ; Tomás, María ; Wood, Thomas K. ; García-Contreras, Rodolfo. / Pyocyanin restricts social cheating in Pseudomonas aeruginosa. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. JUN.
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abstract = "Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.",
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Castañeda-Tamez, P, Ramírez-Peris, J, Pérez-Velázquez, J, Kuttler, C, Jalalimanesh, A, Saucedo-Mora, M, Jiménez-Cortés, JG, Maeda, T, González, Y, Tomás, M, Wood, TK & García-Contreras, R 2018, 'Pyocyanin restricts social cheating in Pseudomonas aeruginosa', Frontiers in Microbiology, vol. 9, no. JUN, 1348. https://doi.org/10.3389/fmicb.2018.01348

Pyocyanin restricts social cheating in Pseudomonas aeruginosa. / Castañeda-Tamez, Paulina; Ramírez-Peris, Jimena; Pérez-Velázquez, Judith; Kuttler, Christina; Jalalimanesh, Ammar; Saucedo-Mora, Miguel; Jiménez-Cortés, J. Guillermo; Maeda, Toshinari; González, Yael; Tomás, María; Wood, Thomas K.; García-Contreras, Rodolfo.

In: Frontiers in Microbiology, Vol. 9, No. JUN, 1348, 27.06.2018.

Research output: Contribution to journalArticle

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T1 - Pyocyanin restricts social cheating in Pseudomonas aeruginosa

AU - Castañeda-Tamez, Paulina

AU - Ramírez-Peris, Jimena

AU - Pérez-Velázquez, Judith

AU - Kuttler, Christina

AU - Jalalimanesh, Ammar

AU - Saucedo-Mora, Miguel

AU - Jiménez-Cortés, J. Guillermo

AU - Maeda, Toshinari

AU - González, Yael

AU - Tomás, María

AU - Wood, Thomas K.

AU - García-Contreras, Rodolfo

PY - 2018/6/27

Y1 - 2018/6/27

N2 - Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.

AB - Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.

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Castañeda-Tamez P, Ramírez-Peris J, Pérez-Velázquez J, Kuttler C, Jalalimanesh A, Saucedo-Mora M et al. Pyocyanin restricts social cheating in Pseudomonas aeruginosa. Frontiers in Microbiology. 2018 Jun 27;9(JUN). 1348. https://doi.org/10.3389/fmicb.2018.01348