Pyroglutamate stimulates Na+-dependent glutamate transport across the blood-brain barrier

Richard A. Hawkins, Ian Simpson, Ashwini Mokashi, Juan R. Viña

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Regulation of Na+-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na+-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be actively removed from brain. γ-Glutamyl transpeptidase, the first enzyme of the γ-glutamyl cycle (GGC), is on the luminal membrane. Pyroglutamate (oxoproline), an intracellular product of GGC, stimulated Na+-dependent transport of glutamate by 46%, whereas facilitative glutamate uptake in luminal membranes was inhibited. This relationship between GGC and glutamate transporters may be part of a regulatory mechanism that accelerates glutamate removal from brain.

Original languageEnglish (US)
Pages (from-to)4382-4386
Number of pages5
JournalFEBS Letters
Volume580
Issue number18
DOIs
StatePublished - Aug 7 2006

Fingerprint

Pyrrolidonecarboxylic Acid
Blood-Brain Barrier
Glutamic Acid
Membranes
Amino Acid Transport System X-AG
Brain
gamma-Glutamyltransferase
Cell membranes
Cell Membrane
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Hawkins, Richard A. ; Simpson, Ian ; Mokashi, Ashwini ; Viña, Juan R. / Pyroglutamate stimulates Na+-dependent glutamate transport across the blood-brain barrier. In: FEBS Letters. 2006 ; Vol. 580, No. 18. pp. 4382-4386.
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Pyroglutamate stimulates Na+-dependent glutamate transport across the blood-brain barrier. / Hawkins, Richard A.; Simpson, Ian; Mokashi, Ashwini; Viña, Juan R.

In: FEBS Letters, Vol. 580, No. 18, 07.08.2006, p. 4382-4386.

Research output: Contribution to journalArticle

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