Pyroglutamate stimulates Na+-dependent glutamate transport across the blood-brain barrier

Richard A. Hawkins, Ian Simpson, Ashwini Mokashi, Juan R. Viña

Research output: Contribution to journalArticle

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Abstract

Regulation of Na+-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na+-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be actively removed from brain. γ-Glutamyl transpeptidase, the first enzyme of the γ-glutamyl cycle (GGC), is on the luminal membrane. Pyroglutamate (oxoproline), an intracellular product of GGC, stimulated Na+-dependent transport of glutamate by 46%, whereas facilitative glutamate uptake in luminal membranes was inhibited. This relationship between GGC and glutamate transporters may be part of a regulatory mechanism that accelerates glutamate removal from brain.

Original languageEnglish (US)
Pages (from-to)4382-4386
Number of pages5
JournalFEBS Letters
Volume580
Issue number18
DOIs
StatePublished - Aug 7 2006

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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