QTL analysis of late blight resistance in a diploid potato family of Solanum phureja x S. stenotomum

S. Costanzo, I. Simko, Barbara Jane Christ, K. G. Haynes

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Field resistance to Phytophthora infestans (Mont.) de Bary, the causal agent of late blight in potatoes, has been characterized in a potato segregating family of 230 full-sib progenies derived from a cross between two hybrid Solanum phureja x S. stenotomum clones. The distribution of area under the disease progress curve values, measured in different years and locations, was consistent with the inheritance of multigenic resistance. Relatively high levels of resistance and transgressive segregations were also observed within this family. A genetic linkage map of this population was constructed with the intent of mapping quantitative trait loci (QTLs) associated with this late blight field resistance. A total of 132 clones from this family were genotyped based on 162 restriction fragment length polymorphism (RFLP) markers. The genome coverage by the map (855.2 cM) is estimated to be at least 70% and includes 112 segregating RFLP markers and two phenotypic markers, with an average distance of 7.7 cM between two markers. Two methods were employed to determine trait-marker association, the non-parametric Kruskal-Wallis test and interval mapping analysis. Three major QTLs were detected on linkage group III, V, and XI, explaining 23, 17, and 10%, respectively, of the total phenotypic variation. The present study revealed the presence of potentially new genetic loci in this diploid potato family contributing to general resistance against late blight. The identification of these QTLs represents the first step toward their introgression into cultivated tetraploid potato cultivars through marker-assisted selection.

Original languageEnglish (US)
Pages (from-to)609-617
Number of pages9
JournalTheoretical and Applied Genetics
Volume111
Issue number3
DOIs
StatePublished - Aug 1 2005

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Solanum tuberosum subsp. andigenum
Solanum
Quantitative Trait Loci
Phytophthora infestans
Solanum tuberosum
Diploidy
quantitative trait loci
diploidy
potatoes
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
Clone Cells
clones
Genetic Linkage
Genetic Loci
Tetraploidy
transgressive segregation
linkage groups
phenotypic variation
marker-assisted selection

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Costanzo, S. ; Simko, I. ; Christ, Barbara Jane ; Haynes, K. G. / QTL analysis of late blight resistance in a diploid potato family of Solanum phureja x S. stenotomum. In: Theoretical and Applied Genetics. 2005 ; Vol. 111, No. 3. pp. 609-617.
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QTL analysis of late blight resistance in a diploid potato family of Solanum phureja x S. stenotomum. / Costanzo, S.; Simko, I.; Christ, Barbara Jane; Haynes, K. G.

In: Theoretical and Applied Genetics, Vol. 111, No. 3, 01.08.2005, p. 609-617.

Research output: Contribution to journalArticle

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