Qualitative real-time PCR SYBR® Green detection of Petri disease fungi

Barrie E. Overton, Elwin L. Stewart, Xinshun Qu, Nancy G. Wenner, Barbara Jane Christ

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Real-time PCR provides a fast, reliable, and cost-effective method for detecting the presence or absence of Petri disease fungi in grapevines. The primer pairs, Pmo1f + Pmo2r, and Pac1f + Pac2r, were designed for species and genus-specific amplification of Phaeomoniella chlamydospora and Phaeoacremonium spp. respectively, using real-time PCR with SYBR® Green. The primers were specific and showed no primer-primer dimers until after 35 cycles. Pa. chlamydospora was detected in roots, shoots, and young trunks of drill-inoculated vines. Phaeoacremonium was detected in trunk cross-sections of naturally infected vines from which Phaeoacremonium aleophilum had been isolated. The protocol presented here can be adapted to provide a reliable detection system for research and industry.

Original languageEnglish (US)
Pages (from-to)403-410
Number of pages8
JournalPhytopathologia Mediterranea
Volume43
Issue number3
StatePublished - Dec 1 2004

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

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