Quantification of choline and ethanolamine phospholipids in rabbit myocardium

Michael Creer, Christine Pastor, Peter B. Corr, Richard W. Gross, Burton E. Sobel

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on 14 C-labeled internal standards.

Original languageEnglish (US)
Pages (from-to)65-74
Number of pages10
JournalAnalytical Biochemistry
Volume144
Issue number1
DOIs
StatePublished - Jan 1 1985

Fingerprint

Ethanolamine
Choline
Phospholipids
Myocardium
Rabbits
High performance liquid chromatography
Chloroform
Phosphorus
Methanol
Ischemia
High Pressure Liquid Chromatography
Tissue
Lipids
Recovery

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Creer, Michael ; Pastor, Christine ; Corr, Peter B. ; Gross, Richard W. ; Sobel, Burton E. / Quantification of choline and ethanolamine phospholipids in rabbit myocardium. In: Analytical Biochemistry. 1985 ; Vol. 144, No. 1. pp. 65-74.
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Quantification of choline and ethanolamine phospholipids in rabbit myocardium. / Creer, Michael; Pastor, Christine; Corr, Peter B.; Gross, Richard W.; Sobel, Burton E.

In: Analytical Biochemistry, Vol. 144, No. 1, 01.01.1985, p. 65-74.

Research output: Contribution to journalArticle

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