Quantification of crystalline cellulose in lignocellulosic biomass using sum frequency generation (SFG) vibration spectroscopy and comparison with other analytical methods

Anna L. Barnette, Christopher Lee, Laura C. Bradley, Edward P. Schreiner, Yong Bum Park, Heenae Shin, Daniel J. Cosgrove, Sunkyu Park, Seong H. Kim

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The non-centrosymmetry requirement of sum frequency generation (SFG) vibration spectroscopy allows the detection and quantification of crystalline cellulose in lignocellulose biomass without spectral interferences from hemicelluloses and lignin. This paper shows a correlation between the amount of crystalline cellulose in biomass and the SFG signal intensity. Model biomass samples were prepared by mixing commercially available cellulose, xylan, and lignin to defined concentrations. The SFG signal intensity was found sensitive to a wide range of crystallinity, but varied non-linearly with the mass fraction of cellulose in the samples. This might be due to the matrix effects such as light scattering and absorption by xylan and lignin, as well as the non-linear density dependence of the SFG process itself. Comparison with other techniques such as XRD, FT-Raman, FT-IR and NMR demonstrate that SFG can be a complementary and sensitive tool to assess crystalline cellulose in biomass.

Original languageEnglish (US)
Pages (from-to)802-809
Number of pages8
JournalCarbohydrate Polymers
Volume89
Issue number3
DOIs
StatePublished - Jul 1 2012

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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