Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS

Christopher J. Smith, Wenlin Huang, Charisse J. Walcott, Wayman Turner, James Grainger, Donald G. Patterson

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

For measurement of biomarkers from polycyclic aromatic hydrocarbon (PAH) exposure, an analytical method is described quantifying hydroxylated PAH (OH-PAH) in urine samples. This method determined monohydroxy metabolites of naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene. The sample preparation consisted of enzymatic hydrolysis, solid-phase extraction and derivatization with a silylating reagent. Five carbon-13 labeled standards were used for isotope dilution. Analytes were separated by gas chromatography (GC) and quantified with high-resolution mass spectrometry (HRMS). This method produced good recoveries (41-70%), linearity, and specificity. Data were corrected for blank levels from the naphthalene, fluorene, and phenanthrene metabolites. Method detection limits ranged from 2 ng L-1 for 1-hydroxypyrene to 43.5 ng L-1 for 1-hydroxynaphthalene. Using quality control charts from two urine pools, the method can be readily applied to biomonitoring PAH exposure.

Original languageEnglish (US)
Pages (from-to)216-220
Number of pages5
JournalFresenius' Journal of Analytical Chemistry
Volume372
Issue number1
DOIs
StatePublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry

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