Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS

Christopher J. Smith, Wenlin Huang, Charisse J. Walcott, Wayman Turner, James Grainger, Donald George Jr Patterson

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

For measurement of biomarkers from polycyclic aromatic hydrocarbon (PAH) exposure, an analytical method is described quantifying hydroxylated PAH (OH-PAH) in urine samples. This method determined monohydroxy metabolites of naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene. The sample preparation consisted of enzymatic hydrolysis, solid-phase extraction and derivatization with a silylating reagent. Five carbon-13 labeled standards were used for isotope dilution. Analytes were separated by gas chromatography (GC) and quantified with high-resolution mass spectrometry (HRMS). This method produced good recoveries (41-70%), linearity, and specificity. Data were corrected for blank levels from the naphthalene, fluorene, and phenanthrene metabolites. Method detection limits ranged from 2 ng L-1 for 1-hydroxypyrene to 43.5 ng L-1 for 1-hydroxynaphthalene. Using quality control charts from two urine pools, the method can be readily applied to biomonitoring PAH exposure.

Original languageEnglish (US)
Pages (from-to)216-220
Number of pages5
JournalFresenius' Journal of Analytical Chemistry
Volume372
Issue number1
StatePublished - Jan 1 2002

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Polycyclic Aromatic Hydrocarbons
Solid Phase Extraction
Metabolites
Isotopes
Gas chromatography
Gas Chromatography
Dilution
Urine
Enzymatic hydrolysis
Biomarkers
Environmental Monitoring
Quality control
Mass spectrometry
Carbon
Quality Control
Limit of Detection
Mass Spectrometry
Recovery
Hydrolysis
phenanthrene

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Smith, C. J., Huang, W., Walcott, C. J., Turner, W., Grainger, J., & Patterson, D. G. J. (2002). Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS. Fresenius' Journal of Analytical Chemistry, 372(1), 216-220.
Smith, Christopher J. ; Huang, Wenlin ; Walcott, Charisse J. ; Turner, Wayman ; Grainger, James ; Patterson, Donald George Jr. / Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS. In: Fresenius' Journal of Analytical Chemistry. 2002 ; Vol. 372, No. 1. pp. 216-220.
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Smith, CJ, Huang, W, Walcott, CJ, Turner, W, Grainger, J & Patterson, DGJ 2002, 'Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS', Fresenius' Journal of Analytical Chemistry, vol. 372, no. 1, pp. 216-220.

Quantification of monohydroxy-PAH metabolites in urine by solid-phase extraction with isotope dilution-GC-MS. / Smith, Christopher J.; Huang, Wenlin; Walcott, Charisse J.; Turner, Wayman; Grainger, James; Patterson, Donald George Jr.

In: Fresenius' Journal of Analytical Chemistry, Vol. 372, No. 1, 01.01.2002, p. 216-220.

Research output: Contribution to journalArticle

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