Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives: An adaptation of previously described methodology

Maria Rita Castro, Eduard A. Struys, Erwin E.W. Jansen, Henk J. Blom, Isabel Tavares de Almeida, Cornelis Jakobs

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18 Scopus citations

Abstract

A simplified reversed phase HPLC system for the detection of fluorescent 1,N6-etheno derivatives of SAM (S-adenosylmethionine) and S-adenosylhomocysteine (SAH) is described. The most important changes from the previously reported method are a shorter derivatization reaction time, the use of a solid-phase extraction resulting in an increase of the method's sensitivity, and the use of only one chromatographic system to separate SAM and SAH (in which the use of an ion-pairing reagent in the mobile phase is avoided). The linearity of the method was established, and the intra-assay coefficients of variation were 10.4 and 4.7% for SAM and SAH, respectively. Normal plasma values (n=8), evaluated with the present methodology, were, for SAM and SAH, respectively, 57±12 and 28±3 nM (mean±SD).

Original languageEnglish (US)
Pages (from-to)963-968
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume29
Issue number5
DOIs
StatePublished - Jul 31 2002

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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