Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives

An adaptation of previously described methodology

Maria Rita Castro, Eduard A. Struys, Erwin E.W. Jansen, Henk J. Blom, Isabel Tavares de Almeida, Cornelis Jakobs

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A simplified reversed phase HPLC system for the detection of fluorescent 1,N6-etheno derivatives of SAM (S-adenosylmethionine) and S-adenosylhomocysteine (SAH) is described. The most important changes from the previously reported method are a shorter derivatization reaction time, the use of a solid-phase extraction resulting in an increase of the method's sensitivity, and the use of only one chromatographic system to separate SAM and SAH (in which the use of an ion-pairing reagent in the mobile phase is avoided). The linearity of the method was established, and the intra-assay coefficients of variation were 10.4 and 4.7% for SAM and SAH, respectively. Normal plasma values (n=8), evaluated with the present methodology, were, for SAM and SAH, respectively, 57±12 and 28±3 nM (mean±SD).

Original languageEnglish (US)
Pages (from-to)963-968
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume29
Issue number5
DOIs
StatePublished - Jul 31 2002

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S-Adenosylhomocysteine
S-Adenosylmethionine
Derivatives
Plasmas
Solid Phase Extraction
Assays
Reference Values
High Pressure Liquid Chromatography
Ions

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

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title = "Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives: An adaptation of previously described methodology",
abstract = "A simplified reversed phase HPLC system for the detection of fluorescent 1,N6-etheno derivatives of SAM (S-adenosylmethionine) and S-adenosylhomocysteine (SAH) is described. The most important changes from the previously reported method are a shorter derivatization reaction time, the use of a solid-phase extraction resulting in an increase of the method's sensitivity, and the use of only one chromatographic system to separate SAM and SAH (in which the use of an ion-pairing reagent in the mobile phase is avoided). The linearity of the method was established, and the intra-assay coefficients of variation were 10.4 and 4.7{\%} for SAM and SAH, respectively. Normal plasma values (n=8), evaluated with the present methodology, were, for SAM and SAH, respectively, 57±12 and 28±3 nM (mean±SD).",
author = "Castro, {Maria Rita} and Struys, {Eduard A.} and Jansen, {Erwin E.W.} and Blom, {Henk J.} and {de Almeida}, {Isabel Tavares} and Cornelis Jakobs",
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Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives : An adaptation of previously described methodology. / Castro, Maria Rita; Struys, Eduard A.; Jansen, Erwin E.W.; Blom, Henk J.; de Almeida, Isabel Tavares; Jakobs, Cornelis.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 29, No. 5, 31.07.2002, p. 963-968.

Research output: Contribution to journalArticle

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T2 - An adaptation of previously described methodology

AU - Castro, Maria Rita

AU - Struys, Eduard A.

AU - Jansen, Erwin E.W.

AU - Blom, Henk J.

AU - de Almeida, Isabel Tavares

AU - Jakobs, Cornelis

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