Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper

Audrey K. Ellerbee, Scott T. Phillips, Adam C. Siegel, Katherine A. Mirica, Andres W. Martinez, Pierre Striehl, Nina Jain, Mara Prentiss, George M. Whitesides

Research output: Contribution to journalArticle

277 Scopus citations

Abstract

This article describes a point-of-care (POC) system - comprising a microfluidic, paper-based analytical device (μ-PAD) and a hand-held optical colorimeter - for quantifying the concentration of analytes in biological fluids. The μ-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of μ-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.

Original languageEnglish (US)
Pages (from-to)8447-8452
Number of pages6
JournalAnalytical chemistry
Volume81
Issue number20
DOIs
StatePublished - Oct 15 2009

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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    Ellerbee, A. K., Phillips, S. T., Siegel, A. C., Mirica, K. A., Martinez, A. W., Striehl, P., Jain, N., Prentiss, M., & Whitesides, G. M. (2009). Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper. Analytical chemistry, 81(20), 8447-8452. https://doi.org/10.1021/ac901307q