Quantifying the effects of inactin vs Isoflurane anesthesia on gastrointestinal motility in rats using dynamic magnetic resonance imaging and spatio-temporal maps

A. C. Ailiani, T. Neuberger, J. G. Brasseur, G. Banco, Y. Wang, N. B. Smith, A. G. Webb

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Anesthetics are commonly applied in animal studies of gastrointestinal (GI) function. Different anesthetics alter smooth-muscle motility in different ways. The aim of this study is to quantify and compare non-invasively with magnetic resonance imaging (MRI) the motility patterns of the rat gut when anesthetized with inactin vs isoflurane anesthetics in the fed state. Methods: Rats were given an oral gavage of MRI contrast agent for improved visualization of the GI tract. Two-dimensional images through the jejunum of the pre- and postanesthetized rat in the fed state were acquired every 168 ms. Image registration, segmentation, and postprocessing algorithms were applied to produce spatio-temporal maps that were used to quantify peristaltic and segmental motions in the jejunum region interspersed between periods of inactivity. Key Results: There were significantly longer periods of inactivity in the rats treated with isoflurane than in those treated with inactin (179.9 ± 22.4 s vs 17.7 ± 10.3 s). The speed of propagation and wavelength of peristalsis, and the frequency and speed of pattern switching of segmental motility, were higher (p < 0.05) in rats treated with inactin. Conclusions & Inferences: Isoflurane and inactin anesthetics produce significantly different motility behavior with the rat's GI tract in the fed state. Isoflurane anesthetic, results in a reduced frequency of occurrence of motility periods and an overall reduced level of motility in comparison with inactin.

Original languageEnglish (US)
Pages (from-to)1477-1486
Number of pages10
JournalNeurogastroenterology and Motility
Volume26
Issue number10
DOIs
StatePublished - Oct 1 2014

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Gastrointestinal Motility
Isoflurane
Anesthesia
Magnetic Resonance Imaging
Anesthetics
Jejunum
Gastrointestinal Tract
Peristalsis
Contrast Media
Smooth Muscle
thiobutabarbital

All Science Journal Classification (ASJC) codes

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

Cite this

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title = "Quantifying the effects of inactin vs Isoflurane anesthesia on gastrointestinal motility in rats using dynamic magnetic resonance imaging and spatio-temporal maps",
abstract = "Background: Anesthetics are commonly applied in animal studies of gastrointestinal (GI) function. Different anesthetics alter smooth-muscle motility in different ways. The aim of this study is to quantify and compare non-invasively with magnetic resonance imaging (MRI) the motility patterns of the rat gut when anesthetized with inactin vs isoflurane anesthetics in the fed state. Methods: Rats were given an oral gavage of MRI contrast agent for improved visualization of the GI tract. Two-dimensional images through the jejunum of the pre- and postanesthetized rat in the fed state were acquired every 168 ms. Image registration, segmentation, and postprocessing algorithms were applied to produce spatio-temporal maps that were used to quantify peristaltic and segmental motions in the jejunum region interspersed between periods of inactivity. Key Results: There were significantly longer periods of inactivity in the rats treated with isoflurane than in those treated with inactin (179.9 ± 22.4 s vs 17.7 ± 10.3 s). The speed of propagation and wavelength of peristalsis, and the frequency and speed of pattern switching of segmental motility, were higher (p < 0.05) in rats treated with inactin. Conclusions & Inferences: Isoflurane and inactin anesthetics produce significantly different motility behavior with the rat's GI tract in the fed state. Isoflurane anesthetic, results in a reduced frequency of occurrence of motility periods and an overall reduced level of motility in comparison with inactin.",
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Quantifying the effects of inactin vs Isoflurane anesthesia on gastrointestinal motility in rats using dynamic magnetic resonance imaging and spatio-temporal maps. / Ailiani, A. C.; Neuberger, T.; Brasseur, J. G.; Banco, G.; Wang, Y.; Smith, N. B.; Webb, A. G.

In: Neurogastroenterology and Motility, Vol. 26, No. 10, 01.10.2014, p. 1477-1486.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantifying the effects of inactin vs Isoflurane anesthesia on gastrointestinal motility in rats using dynamic magnetic resonance imaging and spatio-temporal maps

AU - Ailiani, A. C.

AU - Neuberger, T.

AU - Brasseur, J. G.

AU - Banco, G.

AU - Wang, Y.

AU - Smith, N. B.

AU - Webb, A. G.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Background: Anesthetics are commonly applied in animal studies of gastrointestinal (GI) function. Different anesthetics alter smooth-muscle motility in different ways. The aim of this study is to quantify and compare non-invasively with magnetic resonance imaging (MRI) the motility patterns of the rat gut when anesthetized with inactin vs isoflurane anesthetics in the fed state. Methods: Rats were given an oral gavage of MRI contrast agent for improved visualization of the GI tract. Two-dimensional images through the jejunum of the pre- and postanesthetized rat in the fed state were acquired every 168 ms. Image registration, segmentation, and postprocessing algorithms were applied to produce spatio-temporal maps that were used to quantify peristaltic and segmental motions in the jejunum region interspersed between periods of inactivity. Key Results: There were significantly longer periods of inactivity in the rats treated with isoflurane than in those treated with inactin (179.9 ± 22.4 s vs 17.7 ± 10.3 s). The speed of propagation and wavelength of peristalsis, and the frequency and speed of pattern switching of segmental motility, were higher (p < 0.05) in rats treated with inactin. Conclusions & Inferences: Isoflurane and inactin anesthetics produce significantly different motility behavior with the rat's GI tract in the fed state. Isoflurane anesthetic, results in a reduced frequency of occurrence of motility periods and an overall reduced level of motility in comparison with inactin.

AB - Background: Anesthetics are commonly applied in animal studies of gastrointestinal (GI) function. Different anesthetics alter smooth-muscle motility in different ways. The aim of this study is to quantify and compare non-invasively with magnetic resonance imaging (MRI) the motility patterns of the rat gut when anesthetized with inactin vs isoflurane anesthetics in the fed state. Methods: Rats were given an oral gavage of MRI contrast agent for improved visualization of the GI tract. Two-dimensional images through the jejunum of the pre- and postanesthetized rat in the fed state were acquired every 168 ms. Image registration, segmentation, and postprocessing algorithms were applied to produce spatio-temporal maps that were used to quantify peristaltic and segmental motions in the jejunum region interspersed between periods of inactivity. Key Results: There were significantly longer periods of inactivity in the rats treated with isoflurane than in those treated with inactin (179.9 ± 22.4 s vs 17.7 ± 10.3 s). The speed of propagation and wavelength of peristalsis, and the frequency and speed of pattern switching of segmental motility, were higher (p < 0.05) in rats treated with inactin. Conclusions & Inferences: Isoflurane and inactin anesthetics produce significantly different motility behavior with the rat's GI tract in the fed state. Isoflurane anesthetic, results in a reduced frequency of occurrence of motility periods and an overall reduced level of motility in comparison with inactin.

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