Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

microRNA (miRNA) mediated repression of target genes plays essential roles in a variety of functions in plants. An easy-to-use method that can effectively validate functional miRNA-target interactions in plants thus is of particular interest. Here, we describe an Agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. With this method, the strength of miRNA-mediated target repression can be quantified at both the mRNA (via qRT-PCR) and protein (via dual-luciferase assay) levels quickly and accurately.

Original languageEnglish (US)
Title of host publicationPlant Functional Genomics
Subtitle of host publicationMethods and Protocols: Second Edition
PublisherSpringer New York
Pages287-303
Number of pages17
ISBN (Electronic)9781493924448
ISBN (Print)9781493924431
DOIs
StatePublished - Mar 11 2015

Fingerprint

luciferase
Luciferases
MicroRNAs
microRNA
Agrobacterium tumefaciens
Essential Genes
assays
Agrobacterium radiobacter
Polymerase Chain Reaction
Messenger RNA
methodology
Proteins
genes
proteins

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Liu, Q., & Axtell, M. J. (2015). Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. In Plant Functional Genomics: Methods and Protocols: Second Edition (pp. 287-303). Springer New York. https://doi.org/10.1007/978-1-4939-2444-8_14
Liu, Qikun ; Axtell, Michael J. / Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, 2015. pp. 287-303
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Liu, Q & Axtell, MJ 2015, Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. in Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, pp. 287-303. https://doi.org/10.1007/978-1-4939-2444-8_14

Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. / Liu, Qikun; Axtell, Michael J.

Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, 2015. p. 287-303.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Liu Q, Axtell MJ. Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. In Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York. 2015. p. 287-303 https://doi.org/10.1007/978-1-4939-2444-8_14