Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

microRNA (miRNA) mediated repression of target genes plays essential roles in a variety of functions in plants. An easy-to-use method that can effectively validate functional miRNA-target interactions in plants thus is of particular interest. Here, we describe an Agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. With this method, the strength of miRNA-mediated target repression can be quantified at both the mRNA (via qRT-PCR) and protein (via dual-luciferase assay) levels quickly and accurately.

Original languageEnglish (US)
Title of host publicationPlant Functional Genomics
Subtitle of host publicationMethods and Protocols: Second Edition
PublisherSpringer New York
Pages287-303
Number of pages17
ISBN (Electronic)9781493924448
ISBN (Print)9781493924431
DOIs
StatePublished - Mar 11 2015

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

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    Liu, Q., & Axtell, M. J. (2015). Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system. In Plant Functional Genomics: Methods and Protocols: Second Edition (pp. 287-303). Springer New York. https://doi.org/10.1007/978-1-4939-2444-8_14