Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system

Research output: Contribution to journalArticle

Abstract

microRNA (miRNA) mediated repression of target genes plays essential roles in a variety of functions in plants. An easy-to-use method that can effectively validate functional miRNA–target interactions in plants thus is of particular interest. Here, we describe an Agrobacterium tumefaciens -mediated in vivo assay utilizing a dual-luciferase reporter system. With this method, the strength of miRNA-mediated target repression can be quantifi ed at both the mRNA (via qRT-PCR) and protein (via dual-luciferase assay) levels quickly and accurately.

Original languageEnglish (US)
Pages (from-to)287-303
Number of pages17
JournalMethods in Molecular Biology
Volume1284
DOIs
Publication statusPublished - Jan 1 2015

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this