microRNA (miRNA) mediated repression of target genes plays essential roles in a variety of functions in plants. An easy-to-use method that can effectively validate functional miRNA–target interactions in plants thus is of particular interest. Here, we describe an Agrobacterium tumefaciens -mediated in vivo assay utilizing a dual-luciferase reporter system. With this method, the strength of miRNA-mediated target repression can be quantifi ed at both the mRNA (via qRT-PCR) and protein (via dual-luciferase assay) levels quickly and accurately.
|Original language||English (US)|
|Number of pages||17|
|Journal||Methods in Molecular Biology|
|State||Published - Jan 1 2015|
All Science Journal Classification (ASJC) codes
- Molecular Biology